Evaluation of Streptomyces strains isolated from herbal vermicompost for their plant growth-promotion traits in rice

Gopalakrishnan, S and Srinivas, V and Prakash, B and Arumugam, S and Vijayabharathi, R and Rupela, O and Kudapa, H and Krishnamohan, K and Varshney, R K (2014) Evaluation of Streptomyces strains isolated from herbal vermicompost for their plant growth-promotion traits in rice. Microbiological Research, 169. pp. 40-48. ISSN 0944-5013

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Abstract

Six actinomycetes, CAI-13, CAI-85, CAI-93, CAI-140, CAI-155 and KAI-180, isolated from six different herbal vermi-composts were characterized for in-vitro plant growth-promoting (PGP) properties and further evaluated in the field for PGP activity in rice. Of the six actinomycetes, CAI-13, CAI-85, CAI-93, CAI-140 and CAI-155 produced siderophores; CAI-13, CAI-93, CAI-155 and KAI-180 produced chitinase; CAI-13, CAI-140, CAI-155 and KAI-180 produced lipase; CAI-13, CAI-93, CAI-155 and KAI-180 produced protease; and CAI-13, CAI-85, CAI-140 and CAI-155 produced β-1-3-glucanase whereas all the six actinomycetes produced cellulase, hydrocyanic acid and indole acetic acid (IAA). The actinomycetes were able to grow in NaCl concentrations of up to 8%, at pH values between 7 and 11, temperatures between 20 and 40 °C and compatible with fungicide bavistin at field application levels. In the rice field, the actinomycetes significantly enhanced tiller numbers, panicle numbers, filled grain numbers and weight, stover yield, grain yield, total dry matter, root length, volume and dry weight over the un-inoculated control. In the rhizosphere, the actinomycetes also significantly enhanced total nitrogen, available phosphorous, % organic carbon, microbial biomass carbon and nitrogen and dehydrogenase activity over the un-inoculated control. Sequences of 16S rDNA gene of the actinomycetes matched with different Streptomyces species in BLAST analysis. Of the six actinomycetes, CAI-85 and CAI-93 were found superior over other actinomycetes in terms of PGP properties, root development and crop productivity. qRT-PCR analysis on selected plant growth promoting genes of actinomycetes revealed the up-regulation of IAA genes only in CAI-85 and CAI-93.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: UNSPECIFIED
Uncontrolled Keywords: Actinomycetes; Streptomyces spp.; Field evaluation; Plant growth-promotion; Rice
Subjects: Others > Fertilizer Applications
Depositing User: Mr Siva Shankar
Date Deposited: 20 Sep 2013 05:02
Last Modified: 27 Sep 2016 04:07
URI: http://oar.icrisat.org/id/eprint/7107
Official URL: http://dx.doi.org/10.1016/j.micres.2013.09.008
Projects: UNSPECIFIED
Funders: National Bureau of Agriculturally ImportantMicroorganisms (NBAIM)
Acknowledgement: UNSPECIFIED
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