Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics

Domoney, C and Knox, M and Moreau , C and Ambrose, M and Palmer, J M and Smith, P and Christodoulou, V and Isaac, P G and Hegarty, M and Blackmore, T and Swain, M and Ellis, N (2013) Exploiting a fast neutron mutant genetic resource in Pisum sativum (pea) for functional genomics. Functional Plant Biology. A-J. ISSN 1445-4408

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Abstract

A fast neutron (FN)-mutagenised population was generated in Pisum sativum L. (pea) to enable the identification and isolation of genes underlying traits and processes. Studies of several phenotypic traits have clearly demonstrated the utility of the resource by associating gene deletions with phenotype followed by functional tests exploiting additional mutant sources, from both induced and natural variant germplasm. For forward genetic screens, next generation sequencing methodologies provide an opportunity for identifying genes associated with deletions rapidly and systematically. The application of rapid reverse genetic screens of the fast neutron mutant pea population supports conclusions on the frequency of deletions based on phenotype alone. These studies also suggest that large deletions affecting one or more loci can be non-deleterious to the pea genome, yielding mutants that could not be obtained by other means. Deletion mutants affecting genes associated with seed metabolism and storage are providing unique opportunities to identify the products of complex and related gene families, and to study the downstream consequences of such deletions.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: fast neutron mutagenesis, genomic deletions, seed proteins.
Subjects: Others > Genetics and Genomics
Depositing User: Mr Siva Shankar
Date Deposited: 29 Aug 2013 06:01
Last Modified: 28 Dec 2013 08:05
URI: http://oar.icrisat.org/id/eprint/7078
Official URL: http://dx.doi.org/10.1071/FP13147
Projects: UNSPECIFIED
Funders: BBSRC (BB/J004561/1), John Innes Foundation, Defra (IF0147, Pulse Crop Genetic Improvement Network), BBSRC/Defra/Technology Strategy Board (TS/J002852/1). NE
Acknowledgement: This paper originates from a presentation at the ‘VI International Conference on Legume Genetics and Genomics (ICLGG)’ Hyderabad, India, 2–7 October 2012.
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