Single Nucleotide Polymorphism–based Genetic Diversity in the Reference Set of Peanut (Arachis spp.) by Developing and Applying Cost-Effective Kompetitive Allele Specific Polymerase Chain Reaction Genotyping Assays

Khera, P and Upadhyaya, H D and Pandey, M K and Roorkiwal, M and Sriswathi, M and Janila, P and Guo, Y and McKain, M R and Nagy, E D and Knapp, S J and Leebens-Mack, J and Conner, J A and Ozias-Akins, P and Varshney, R K (2013) Single Nucleotide Polymorphism–based Genetic Diversity in the Reference Set of Peanut (Arachis spp.) by Developing and Applying Cost-Effective Kompetitive Allele Specific Polymerase Chain Reaction Genotyping Assays. The Plant Genome, 6 (3). pp. 1-11. ISSN 1940-3372

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Abstract

Kompetitive allele-specific polymerase chain reaction (KASP) assays have emerged as cost-effective marker assays especially for molecular breeding applications. Therefore, a set of 96 informative single nucleotide polymorphisms (SNPs) was used to develop KASP assays in groundnut or peanut (Arachis spp.). Developed assays were designated as groundnut KASP assay markers (GKAMs) and screened on 94 genotypes (validation set) that included parental lines of 27 mapping populations, seven synthetic autotetraploid and amphidiploid lines, and 19 wild species accessions. As a result, 90 GKAMs could be validated and 73 GKAMs showed polymorphism in the validation set. Validated GKAMs were screened on 280 diverse genotypes of the reference set for estimating diversity features and elucidating genetic relationships. Cluster analysis of marker allelic data grouped accessions according to their genome type, subspecies, and botanical variety. The subspecies Arachis hypogaea L. subsp. fastigiata Waldron and A. hypogaea subsp. hypogaea formed distinct cluster; however, some overlaps were found indicating their frequent intercrossing during the course of evolution. The wild species, having diploid genomes, were grouped into a single cluster. The average polymorphism information content value for polymorphic GKAMs was 0.32 in the validation set and 0.31 in the reference set. These validated and highly informative GKAMs may be useful for genetics and breeding applications in Arachis species.

Item Type: Article
Divisions: UNSPECIFIED
CRPS: UNSPECIFIED
Subjects: Mandate crops > Groundnut
Others > Genetics and Genomics
Depositing User: Mr Siva Shankar
Date Deposited: 13 Aug 2013 03:32
Last Modified: 30 Jan 2014 11:10
URI: http://oar.icrisat.org/id/eprint/7049
Official URL: http://dx.doi.org/10.3835/plantgenome2013.06.0019
Projects: CGIAR Research Program on Grain Legumes
Funders: CGIAR Generation Challenge Programme (Theme Leader Discretionary Grant), The Bill & Melinda Gates Foundation (Tropical Legumes I & II)
Acknowledgement: UNSPECIFIED
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