Jayakumar, T
(2010)
Detection of Polymorphism in parental Genotypes of selected mapping Populations in Groundnut(Arachis hypogaea L.).
Masters thesis, Tamil Nadu Agricultural University.
Supervisors
Supervisors Name | Supervisors ID |
---|
Kalamani, A | UNSPECIFIED |
Varshiney, R K | UNSPECIFIED |
Abstract
Availability of polymorphic markers in a crop species is prequisite to construct high density genetic linkage map and mapping of quantitative trait Loci (QTL) and genetic improvement through marker assisted breeding can be achieved. Lack of sufficient molecular markers hinder current genetic research in groundnut (Arachis hypogaea.L.), an allotetraploid species. It is necessary to identify more polymorphic molecular markers for potential use in groundnut genomics. The present study was carried out at M. S. Swaminathan Applied Genomics Laboratory, ICRISAT, Patancheru, India during 2009-2010,to screen the newly designed 1152 BAC-end SSRs among sixteen cultivated groundnut genotypes, which represents the parents of twelve mapping populations. A set of 876 (76 per cent) primer pairs yielded scorable amplicons and 184 (16 per cent) primer pairs showed polymorphism among sixteen parental genotypes. The 184 SSR markers detected 2 to14 alleles with
an average of 3.73 alleles per locus.
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