Ngugi, K and Kimani, W and Kiambi, D and Mutitu, E W (2013) Improving Drought Tolerance in Sorghum bicolor L. Moench: Marker-Assisted Transfer of the Stay-Green Quantitative Trait Loci (QTL) from a Characterized Donor Source into a Local Farmer Variety. International Journal of Scientific Research in Knowledge, 1 (6). pp. 154-162. ISSN 2322-4541
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Abstract
Drought stress is a major constraint to sorghum production in Kenya, especially during flowering stage. This study aimed at developing drought tolerant sorghum varieties by transferring the stay green trait that confers drought tolerance in sorghum from a mapped and characterized donor source into an adapted farmer preferred variety. The drought tolerance donor source, E36-1 originally from Ethiopia was backcrossed into a Kenyan farmer-preferred variety, Ochuti until BC2F1 generation and the stay-green Quantitative Trait Loci (QTL) were transferred through Marker Assisted Breeding (MAB) strategy. Five polymorphic Simple Sequence Repeat (SSR) markers were used to select the 3 stay green QTL of E36-1 found in SBI-01, SBI-07 and SBI-10 linkage groups. In the F1 generation, two of these QTL, were transferred into three genotypes. In the BC1F1 generation, 32 genotypes had at least one QTL incorporated. From a population of 157 BC2F1 progenies, 45 genotypes had incorporated either one or two of the stay-green QTL. Despite a few number of genotypes obtained through the backcrosses, the results showed that stay-green QTL and consequently drought tolerance can be transferred successfully into farmer preferred sorghum varieties through MAB.
| Item Type: | Article |
|---|---|
| Divisions: | RP-Dryland Cereals |
| CRP: | CGIAR Research Program on Dryland Cereals |
| Subjects: | Mandate crops > Sorghum |
| Depositing User: | Mr Sanat Kumar Behera |
| Date Deposited: | 23 Jul 2013 03:19 |
| Last Modified: | 19 Oct 2016 08:06 |
| URI: | http://oar.icrisat.org/id/eprint/6950 |
| Official URL: | http://dx.doi.org/10.12983/ijsrk-2013-p154-162 |
| Projects: | BIOEARN program |
| Funders: | Swedish International Development Cooperation Agency |
| Acknowledgement: | The authors would like to thank, BIOEARN program of SIDA Sweden, through the Inter-University Council of Eastern Africa (IUCEA) for the award of the grant to conduct the research, the University of Nairobi (UoN) for giving them the time to do this work and Biosciences, eastern and central Africa (BecA) and the International Crops Research institute for the Semi-Arid Tropics (ICRISAT) for technical backstopping. |
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