Molecular mapping of genomic regions harbouring QTLs for root and yield traits in sorghum (Sorghum bicolor L. Moench)

Rajkumar, - and Fakurdin, B and Kavil, S P and Girma, Y and Arun, S S and Dadakhalandar, D and Gurusiddesh, B H and Patil, A and Thudi, M and Bhairappanavar, S B and Narayana, Y D and Krishnaraj, P U and Khadi, B M and Kamatar, M Y (2013) Molecular mapping of genomic regions harbouring QTLs for root and yield traits in sorghum (Sorghum bicolor L. Moench). Physiology and Molecular Biology of Plants . pp. 1-11. ISSN 0974-0430

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Abstract

Root system is a vital part of plants for absorbing soil moisture and nutrients and it influences the drought tolerance. Identification of the genomic regions harbouring quantitative trait loci (QTLs) for root and yield traits, and the linked markers can facilitate sorghum improvement through marker-assisted selection (MAS) besides the deeper understanding of the plant response to drought stress. A population of 184 recombinant inbred lines (RILs), derived from E36-1 × SPV570, along with parents were phenotyped for component traits of yield in field and root traits in an above ground rhizotron. High estimates of heritability and genetic advance for all the root traits and for most of the yield traits, presents high scope for improvement of these traits by simple selection. A linkage map constructed with 104 marker loci comprising 50 EST-SSRs, 34 non-genic nuclear SSRs and 20 SNPs, and QTL analysis was performed using composite interval mapping (CIM) approach. A total of eight and 20 QTLs were mapped for root and yield related traits respectively. The QTLs for root volume, root fresh weight and root dry weight were found co-localized on SBI-04, supported by a positive correlation among these traits. Hence, these traits can be improved using the same linked markers. The lack of overlap between the QTLs of component traits of root and yield suggested that these two sets of parameters are independent in their influence and the possibility of combining these two traits might enhance productivity of sorghum under receding moisture condition.

Item Type: Article
Divisions: RP-Grain Legumes
CRP: CGIAR Research Program on Dryland Cereals
Uncontrolled Keywords: Molecular mapping, Genomic regions, QTLs, Sorghum, Sorghum bicolor L. Moench
Subjects: Mandate crops > Sorghum
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 04 Jul 2013 03:17
Last Modified: 19 Oct 2016 08:04
URI: http://oar.icrisat.org/id/eprint/6917
Official URL: http://dx.doi.org/10.1007/s12298-013-0188-0
Projects: UNSPECIFIED
Funders: Government of India - Department of Biotechnology , Government of India - Ministry of Science and Technology
Acknowledgement: This research work was supported by Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India (DBT Programme Support Project). We thank the Project Monitoring and Mentoring Committee (PMMC) members, Dr. V. P. Gupta, Dr. N. Seetharama; Dr. P. Balasubramaniam, Dr. M. B. Chetti, Dr H.E. Shashidhar and Dr. Shailaja Hittalmani for their helpful suggestions from time to time. We acknowledge the timely help of Dr. B. T. Ninganur, in facilitating root studies.
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