Analysis of Genetic Diversity in Pongamia [Pongamia pinnata (L)Pierrre] using AFLP Markers

Thudi, M and Manthena, R and Wani, S P and Tatikonda, L and Hoisington, D A and Varshney, R K (2010) Analysis of Genetic Diversity in Pongamia [Pongamia pinnata (L)Pierrre] using AFLP Markers. Journal of Plant Biochemistry and Biotechnlogy, 19 (2). pp. 209-216.

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Abstract

In recent years, Pongamia has been considered as important renewable source of biodiesel, however not much molecular information is available in this species. Molecular characterization of this legume tree will enhance our understanding in improving the optimal yields of oil through breeding and enable us to meet the future demands for biodiesel. To assess the molecular genetic diversity in 48 Pongamia pinnata accessions collected from six different states of India, amplified fragment length polymorphism (AFLP) marker system was employed. Five AFLP primer combinations produced 520 discernible fragments, of which 502 (96.5%) were polymorphic. AFLP primer informativeness was estimated evaluating four parameters namely polymorphism information content (PIC), effective multiplex ratio (EMR), marker index (MI) and resolving power (RP). In total, 51 unique fragments were detected of which 19 unique fragments were observed with primer combination E-ACG / M-CTA. Although neighbour joining (NJ) method did not group accessions strictly according to their region of collection, a good level of genetic diversity was observed in examined germplasm. However, accessions collected from Karnataka showed comparatively higher diversity than accessions from other states. The diverse accessions identified in this study may be useful in Pongamia pinnata improvement to meet the future demands of biodiesel.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Pongamia pinnata, AFLP, molecular diversity, biofuel, polymorphism
Agro Tags: <b>Agrotags</b> - genetic techniques | genetics | enterprises | pongamia pinnata | polymorphism | information needs | planting | germplasm | oils | fruits <br><b>Fishtags</b> - NOT-AVAILABLE<br><b>Geopoliticaltags</b> - karnataka | andhra pradesh | india | act | haryana | goa | maharashtra | orissa | mexico
Subjects: Others > Oil seeds
Depositing User: Siva Shankar
Date Deposited: 08 Jun 2011 10:44
Last Modified: 31 Jan 2012 05:47
URI: http://oar.icrisat.org/id/eprint/69
Official URL:
Projects: UNSPECIFIED
Funders: Government of India - Department of Biotechnology
Acknowledgement: This work, in part, was undertaken as a part of research activities of Center of Excellence in Genomics (www.icrisat.org/ceg) funded by Department of Biotechnology, Government of India. Thanks are due to Mr A Gafoor, Research Technician, Mr Ch Srinivasa Rao and Ms Seetha Kannan for their help. We gratefully acknowledge the help of Mr A R Nayeemulla, Sustainable Transformation of Rural Areas (SUTRA), Bangalore for sharing the germplasm with us. Authors are also thankful to Dr Reyaz Rouf Mir, ICRISAT and two anonymous reviewers for their valuable suggestions on the MS.
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