Genotype and Growing Environment Interaction Shows a Positive Correlation between Substrates of Raffinose Family Oligosaccharides (RFO) Biosynthesis and Their Accumulation in Chickpea (Cicer arietinum L.) Seeds

Gangola, M P and Khedikar, Y P and Gaur, P M and Baga, M and Chibbar, R N (2013) Genotype and Growing Environment Interaction Shows a Positive Correlation between Substrates of Raffinose Family Oligosaccharides (RFO) Biosynthesis and Their Accumulation in Chickpea (Cicer arietinum L.) Seeds. Journal of Agricultural and Food Chemistry, 61. pp. 4943-4952. ISSN 1520-5118

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Abstract

To develop genetic improvement strategies to modulate raffinose family oligosaccharides (RFO) concentration in chickpea (Cicer arietinum L.) seeds, RFO and their precursor concentrations were analyzed in 171 chickpea genotypes from diverse geographical origins. The genotypes were grown in replicated trials over two years in the field (Patancheru, India) and in the greenhouse (Saskatoon, Canada). Analysis of variance revealed a significant impact of genotype, environment, and their interaction on RFO concentration in chickpea seeds. Total RFO concentration ranged from 1.58 to 5.31 mmol/100 g and from 2.11 to 5.83 mmol/100 g in desi and kabuli genotypes, respectively. Sucrose (0.60−3.59 g/100 g) and stachyose (0.18−2.38 g/ 100 g) were distinguished as the major soluble sugar and RFO, respectively. Correlation analysis revealed a significant positive correlation between substrate and product concentration in RFO biosynthesis. In chickpea seeds, raffinose, stachyose, and verbascose showed a moderate broad sense heritability (0.25−0.56), suggesting the use of a multilocation trials based approach in chickpea seed quality improvement programs.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: chickpea, Cicer arietinum, raffinose family oligosaccharides (RFO), myo-inositol, galactinol, raffinose, stachyose, verbascose, genotype × environment (G × E)
Subjects: Mandate crops > Chickpea
Depositing User: Mr Siva Shankar
Date Deposited: 10 Jun 2013 03:09
Last Modified: 24 Jun 2013 06:12
URI: http://oar.icrisat.org/id/eprint/6845
Official URL: http://dx.doi.org/10.1021/jf3054033
Projects: UNSPECIFIED
Funders: Saskatchewan Pulse Growers (SPG), Natural Sciences and Engineering Research Council (NSERC−CRDPJ collaborative research program), Canada Research Chairs Program, Agriculture and Agri-Food Canada Internationalization program
Acknowledgement: The core research grant of International Crops Research Institute for Semi Arid Tropics (ICRISAT) a CGIAR institute is acknowledged for germplasm development and field experiments.
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