Identification of expressed resistance gene analogs from peanut (Arachis hypogaea L.) expressed sequence tags

Liu, Z and Feng, S and Pandey, M K and Chen, X and Culbreath, A K and Varshney, R K and Guo, B (2013) Identification of expressed resistance gene analogs from peanut (Arachis hypogaea L.) expressed sequence tags. Journal of Integrative Plant Biology, 55 (5). pp. 453-461. ISSN 1744-7909 (In Press)

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Abstract

Low genetic diversity makes peanut (Arachis hypogaea L.) very vulnerable to plant pathogens, causing severe yield loss and reduced seed quality. Several hundred partial genomic DNA sequences as nucleotide-binding-site leucine-rich repeat (NBS-LRR) resistance genes (R) have been identified, but a small portion with expressed transcripts has been found. We aimed to identify resistance gene analogs (RGAs) from peanut expressed sequence tags (ESTs) and to develop polymorphic markers. The protein sequences of 54 known R genes were used to identify homologs from peanut ESTs from public databases. A total of 1,053 ESTs corresponding to six different classes of known R genes were recovered, and assembled 156 contigs and 229 singletons as peanut-expressed RGAs. There were 69 that encoded for NBS-LRR proteins, 191 that encoded for protein kinases, 82 that encoded for LRR-PK/transmembrane proteins, 28 that encoded for Toxin reductases, 11 that encoded for LRR-domain containing proteins and 4 that encoded for TM-domain containing proteins. Twenty-eight simple sequence repeats (SSRs) were identified from 25 peanut expressed RGAs. One SSR polymorphic marker (RGA121) was identified. Two PCR-based markers (Ahsw-1 and Ahsw-2) developed from RGA013 were homologous to the Tomato Spotted Wilt Virus (TSWV) resistance gene. All three markers were mapped on the same linkage group AhIV. These expressed RGAs are the source for RGA-tagged marker development and identification of peanut resistance genes.

Item Type: Article
Divisions: UNSPECIFIED
CRPS: UNSPECIFIED
Uncontrolled Keywords: Arachis hypogaea; expressed sequence tags; resistance gene analogs; TSWV
Subjects: Mandate crops > Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 12 Feb 2013 06:19
Last Modified: 12 Apr 2016 07:54
URI: http://oar.icrisat.org/id/eprint/6561
Official URL: http://dx.doi.org/10.1111/jipb.12037
Projects: UNSPECIFIED
Funders: Georgia Agricultural Commodity Commission, National Peanut Board, Peanut Foundation
Acknowledgement: UNSPECIFIED
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