Genetic dissection of grain weight in bread wheat through quantitative trait locus interval and association mapping

Mir, R R and Kumar, N and Jaiswal, V and Girdharwal, N and Prasad, M and Balyan, H S and Gupta, P K (2012) Genetic dissection of grain weight in bread wheat through quantitative trait locus interval and association mapping. Molecular Breeding, 29 (4). pp. 963-972. ISSN 1380-3743

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Abstract

Genetic dissection of grain weight in bread wheat was undertaken through both genome-wide quantitative trait locus (QTL) interval mapping and association mapping. QTL interval mapping involved preparation of a framework linkage map consisting of 294 loci {194 simple sequence repeats (SSRs), 86 amplified fragment length polymorphisms (AFLPs) and 14 selective amplifications of microsatellite polymorphic loci (SAMPL)} using a bi-parental recombinant inbred line (RIL) mapping population derived from Rye Selection111 × Chinese Spring. Using the genotypic data and phenotypic data on grain weight (GW) of RILs collected over six environments, genome-wide single locus QTL analysis was conducted to identify main effect QTL. This led to identification of as many as ten QTL including four major QTL (three QTL were stable), each contributing >20% phenotypic variation (PV) for GW. The above study was supplemented with association mapping, which allowed identification of 11 new markers in the genomic regions that were not reported earlier to harbour any QTL for GW. It also allowed identification of closely linked markers for six known QTL, and validation of eight QTL reported earlier. The QTL identified through QTL interval mapping and association mapping may prove useful in marker-assisted selection (MAS) for the development of cultivars with high GW in bread wheat

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Bread wheat; Grain weight; QTL interval mapping; Association mapping
Subjects:	Others > Wheat
Depositing User:	Ms K Syamalamba
Date Deposited:	25 Jan 2013 07:45
Last Modified:	25 Jan 2013 07:45
URI:	http://oar.icrisat.org/id/eprint/6469
Official URL:	http://dx.doi.org/10.1007/s11032-011-9693-4
Projects:	UNSPECIFIED
Funders:	Department of Biotechnology (DBT), Government of India for providing financial support to carry out this study. Financial support was also received from the DST through FIST-program and from the University Grants Commission (UGC), New Delhi
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

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