Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton

Srinivasa Rao, P and Kumar, P S and Sonti, R V (2013) Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton. Journal of Biosciences, 38 (1). pp. 167-172. ISSN 0250-5991

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Abstract

Genomic DNA isolation in cotton is complicated because of the presence of secondary metabolites that are inhibitory to PCR amplification. We report here that radicle tips, but not other parts of cotton seedlings, yield high-quality DNA that is readily amenable for PCR. The radicle-tip-excised seedlings retain viability because of the formation of adventitious roots. We demonstrate the utility of this method in distinguishing homozygotes from heterozygotes in a cotton breeding population and in hybrid seed purity testing

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Cotton; cry1Ac; genomic DNA isolation; high-resolution melting curve analysis; radicle tip; seed purity testing
Subjects: Others > Genetics and Genomics
Depositing User: Ms K Syamalamba
Date Deposited: 25 Jan 2013 04:51
Last Modified: 07 Feb 2014 05:10
URI: http://oar.icrisat.org/id/eprint/6467
Official URL: http://dx.doi.org/10.1007/s12038-012-9285-4
Projects: UNSPECIFIED
Funders: Government of India - Department of Biotechnology
Acknowledgement: UNSPECIFIED
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