Use of random amplified polymorphic DNA markers for mapping the chickpea genome

Banerjee, H and Pai, R A and Moss, J P and Sharma, R P (2001) Use of random amplified polymorphic DNA markers for mapping the chickpea genome. Biologia Plantarum, 44 (2). pp. 195-202. ISSN 0006-3134

PDF - Published Version Restricted to ICRISAT users only Download (583kB) | Request a copy

Abstract

Three interspecific crosses were developed using Cicer arietinum (ICC 4918) as the female parent and wild Cicer species [C. reticulatum - JM 2100, JM 2106 and C. echinospermum - ICCW 44] as the male parent. Cicer arietinum (ICC 4918) × C. reticulatum (JM 2100) cross produced the largest number of F2 plants and was chosen for linkage mapping using Random Amplified Polymorphic DNA (RAPD) primers. A partial linkage map was constructed based upon the segregation of 36 RAPD markers obtained by amplification using 35 primers. The linkage map consists of two linkage groups with 17 linked markers covering a total of 464.9 cM. Analyses also revealed association of three morphological traits with linked RAPD markers. Out of seven morphological traits tested for association with linked markers in the segregating plants, four Quantitative trait loci (QTL) were detected for the trait leaf length and three QTLs each for the traits leaf width and erect plant habit

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Cicer arietinum; Cicer reticulatum; Cicer echinospermum; RAPD; molecular linkage map; quantitative trait loci
Subjects:	Mandate crops > Chickpea
Depositing User:	Ms K Syamalamba
Date Deposited:	23 Jan 2013 10:34
Last Modified:	23 Jan 2013 10:34
URI:	http://oar.icrisat.org/id/eprint/6459
Official URL:	http://dx.doi.org/10.1023/A:1010234922360
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Google Scholar

View Statistics

Actions (login required)

View Item