Random amplified polymorphic DNA variation within and among bean landrace mixtures (Phaseolus vulgaris L.) from Tanzania

Briand, L and Brown, A E and Lenné, J M and Teverson, D M (1998) Random amplified polymorphic DNA variation within and among bean landrace mixtures (Phaseolus vulgaris L.) from Tanzania. Euphytica, 102 (3). pp. 371-377. ISSN 0014-2336

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Abstract

Genetic characterization of 51 individual pure lines from 13 landraces of three common bean (Phaseolus vulgaris L.) mixtures from the southern highlands of Tanzania was undertaken using random amplified polymorphic DNA (RAPD) analysis. A dendrogram generated by cluster analysis from data derived from fragments amplified by 12 random 10-base primers divided the bean individuals onto two main branches with less than 60% genetic similarity. Branches A and B subdivided into two and four clusters, respectively. Mixture 2, comprising three landraces, was the most uniform, most plants appearing on cluster 4 of branch B. Three of the four landraces of mixture 1 appeared on cluster 3 of branch B while the fourth landrace appeared on major branch A. Mixture 3 showed the greatest genetic variation with components appearing on both major branches. The clear separation of the 13 landraces onto two main branches of the dendrogram together with phenotypic characters, notably variation in bean size, suggests that the two groups might represent two distinct gene pools of P. vulgaris

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Phaseolus vulgaris; common bean; landraces; genetic diversity; RAPD analysis
Subjects: Others > Genetics and Genomics
Depositing User: Ms K Syamalamba
Date Deposited: 18 Jan 2013 08:56
Last Modified: 07 Feb 2014 06:22
URI: http://oar.icrisat.org/id/eprint/6439
Official URL: http://dx.doi.org/10.1023/A:1018365818566
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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