Integrated Consensus Map of Cultivated Peanut and Wild Relatives Reveals Structures of the A and B Genomes of Arachis and Divergence of the Legume Genomes

Shirasawa, K and Bertioli, D J and Varshney, R K and Moretzsohn, M C and Leal-Bertioli, S C M and Thudi, M and Pandey, M K and Rami, Jean-François and Foncéka, D and Gowda, M V C and Qin, H and Guo, B and Hong, Y and Liang, X and Hirakawa, H and Tabata, S and Isobe, S (2013) Integrated Consensus Map of Cultivated Peanut and Wild Relatives Reveals Structures of the A and B Genomes of Arachis and Divergence of the Legume Genomes. DNA Research, 20 (2). pp. 173-184. ISSN 1756-1663

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Abstract

The complex, tetraploid genome structure of peanut (Arachis hypogaea) has obstructed advances in genetics and genomics in the species. The aim of this study is to understand the genome structure of Arachis by developing a high-density integrated consensus map. Three recombinant inbred line populations derived from crosses between the A genome diploid species, Arachis duranensis and Arachis stenosperma; the B genome diploid species, Arachis ipaënsis and Arachis magna; and between the AB genome tetraploids, A. hypogaea and an artificial amphidiploid (A. ipaënsis × A. duranensis)4×, were used to construct genetic linkage maps: 10 linkage groups (LGs) of 544 cM with 597 loci for the A genome; 10 LGs of 461 cM with 798 loci for the B genome; and 20 LGs of 1442 cM with 1469 loci for the AB genome. The resultant maps plus 13 published maps were integrated into a consensus map covering 2651 cM with 3693 marker loci which was anchored to 20 consensus LGs corresponding to the A and B genomes. The comparative genomics with genome sequences of Cajanus cajan, Glycine max, Lotus japonicus, and Medicago truncatula revealed that the Arachis genome has segmented synteny relationship to the other legumes. The comparative maps in legumes, integrated tetraploid consensus maps, and genome-specific diploid maps will increase the genetic and genomic understanding of Arachis and should facilitate molecular breeding.

Item Type: Article
Divisions: UNSPECIFIED
CRPS: UNSPECIFIED
Uncontrolled Keywords: Arachis spp.; comparative genomics; genetic linkage map; integrated consensus map; legume genome
Subjects: Mandate crops > Groundnut
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 14 Jan 2013 04:10
Last Modified: 06 Aug 2013 05:03
URI: http://oar.icrisat.org/id/eprint/6409
Official URL: http://dx.doi.org/10.1093/dnares/dss042
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We are grateful to S. Sasamoto, S. Nakayama, A. Watanabe, T. Fujishiro, M. Kato, Y. Kishida, M. Kohara, C. Minami, K. Nanri, C. Takahashi, and H. Tsuruoka in the Kazusa DNA Research Institute for their technical assistance
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