Dissecting tocopherols content in maize (Zea mays L.), using two segregating populations and high-density single nucleotide polymorphism markers

Shutu, X and Dalong, Z and Ye, C and Yi, Z and Shah, T and et al, . (2012) Dissecting tocopherols content in maize (Zea mays L.), using two segregating populations and high-density single nucleotide polymorphism markers. BMC Plant Biology, 12 (201). pp. 1-28. ISSN 1471-2229

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Abstract

Two F2 populations (K22/CI7 and K22/Dan340, referred to as POP-1 and POP-2, respectively), which share a common parent (K22), were developed and genotyped using a GoldenGate assay containing 1,536 single nucleotide polymorphism (SNP) markers. An integrated genetic linkage map was constructed using 619 SNP markers, spanning a total of 1649.03 cM of the maize genome with an average interval of 2.67 cM. Seventeen quantitative trait loci (QTLs) for all the traits were detected in the first map and 13 in the second. In these two maps, QTLs for different traits were localized to the same genomic regions and some were co-located with candidate genes in the tocopherol biosynthesis pathway. Single QTL was responsible for 3.03% to 52.75% of the phenotypic variation and the QTLs in sum explained23.4% to 66.52% of the total phenotypic variation. A major QTL (qc5-1/qd5-1) affecting alpha-tocopherol (alphaT) was identified on chromosome 5 between the PZA03161.1 and PZA02068.1 in the POP-2. The QTL region was narrowed down from 18.7 Mb to 5.4 Mb by estimating the recombination using high-density markers of the QTL region. This allowed the identification of the candidate gene VTE4 which encodes gamma-tocopherol methyltransferase, an enzyme that transforms gamma-tocopherol (gammaT)to alphaT.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Maize, Tocopherols, QTL mapping
Subjects: Others > Plant Pathology
Others > Maize
Depositing User: Mr Siva Shankar
Date Deposited: 06 Nov 2012 03:49
Last Modified: 06 Nov 2012 03:50
URI: http://oar.icrisat.org/id/eprint/6205
Official URL: http://www.biomedcentral.com/1471-2229/12/201
Projects: UNSPECIFIED
Funders: National Hi- Tech Research and Development Program of China (2012AA10A307), Natural science the National Natural Science Foundation of China (31222041)
Acknowledgement: We would like to thank Abhishek Rathore for help with the construction of the linkage maps. We would also like to thank Santosh Deshpande and Weiwei Wen for their valuable comments in improving this manuscript. Additional file 1: 9036801867708409_add1.rtf, 15299K http://www.biomedcentral.com/imedia/6367416398399653/supp1.rtf
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