Genetic Diversity in Maintainer and Restorer Lines of Pearl Millet

Nepolean, T and Gupta, S K and Dwivedi, S L and Bhattacharjee, R and Rai, K N and Hash, C T (2012) Genetic Diversity in Maintainer and Restorer Lines of Pearl Millet. Crop Science, 52 (6). pp. 2555-2563. ISSN 0011-183X

[img] PDF - Published Version
Restricted to ICRISAT users only

Download (1MB) | Request a copy

Abstract

Molecular markers facilitate rapid and environment-neutral characterization of the pattern of genetic diversity. The International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) at Patancheru, India, has developed a large number and diverse range of maintainer lines (B-lines) and restorer lines (R-lines) of pearl millet [Pennisetum glaucum (L.) R. Br.] in last three decades. In the present study, 213 lines (98 B-lines and 115 R-lines) were genotyped using high throughput assay and 38 polymorphic simple sequence repeat (SSR) markers, which detected 308 alleles averaging 8.1 alleles per locus. Restorer lines were genetically more diverse than B-lines, as higher average gene diversity was detected among R-lines (0.62) than among B-lines (0.50). A neighbor-joining tree based on simple matching dissimilarity distance matrix clearly differentiated B-lines from R-lines into two clusters, with further subdivision of B-lines into four subclusters and R-lines into five subclusters. Seven B-lines clustered with R-lines, and only two R-lines clustered with B-lines. Few unique alleles (1 to 3) were detected in 14 B- and 30 R-lines, which could be used to distinguish them from each other as well as from the remaining lines used in this study. The SSR alleles of each of the parental lines along with distinctness, uniformity, and stability (DUS) characterization may enable ICRISAT to protect these lines from any kind of infringement by the multiple users who have received these lines for hybrid development.

Item Type: Article
Divisions: UNSPECIFIED
CRPS: UNSPECIFIED
Subjects: Mandate crops > Millets
Others > Genetics and Genomics
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 11 Oct 2012 03:41
Last Modified: 11 Oct 2012 03:41
URI: http://oar.icrisat.org/id/eprint/6180
Official URL: http://dx.doi.org/10.2135/cropsci2011.11.0597
Projects: Molecular diversity in relation to morphological diversity and yield heterosis in sorghum and pearl millet
Funders: ICRISAT-Sehgal Family Foundation Endowment Fund
Acknowledgement: Authors acknowledge the support provided by the ICRISATSehgal Family Foundation Endowment Fund to conduct this study, as a part of project (YSFF06) “Molecular diversity in relation to morphological diversity and yield heterosis in sorghum and pearl millet.”
Links:

Actions (login required)

View Item View Item