Pathogen-derived resistance using a viral nucleocapsid gene confers only partial non-durable protection in peanut against peanut bud necrosis virus

Rao, S C and Bhatnagar-Mathur, P and Kumar, P L and Sudarshan Reddy , A and Sharma, K K (2013) Pathogen-derived resistance using a viral nucleocapsid gene confers only partial non-durable protection in peanut against peanut bud necrosis virus. Archives of Virology, 158 (1). pp. 133-143. ISSN 1432-8798

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Abstract

Genetic engineering of peanut (Arachis hypogaea L.) using the gene encoding for the nucleocapsid protein (N gene) of peanut bud necrosis virus (PBNV; genus Tospovirus, family Bunyaviridae) was used to impart resistance to bud necrosis disease in peanut (PBND), a disease for which no durable resistance is available in the existing germplasm. Over 200 transgenic lines of peanut var. JL 24 were developed for which integration and expression of the transgenes was confirmed by PCR, Southern hybridization, RT-PCR and western blot analysis. The T1 and T2 generation transgenic plants were assayed through virus challenge in the greenhouse by using mechanical sap inoculation at 1:100 and 1:50 dilutions of PBNV, and they showed varying levels of disease incidence and intensity. Greenhouse and field evaluation with T2 generation plants indicated somewhat superior performance of the three transgenic events that showed considerable reduction in disease incidence. However, only one of these events showed over 75 % reduction in disease incidence when compared to the untransformed control, indicating partial and non-durable resistance to PBND using the viral N-gene

Item Type: Article
Divisions: UNSPECIFIED
CRPS: UNSPECIFIED
Subjects: Mandate crops > Groundnut
Others > Genetics and Genomics
Others > Entomology
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 29 Sep 2012 06:18
Last Modified: 10 Jan 2014 10:38
URI: http://oar.icrisat.org/id/eprint/6157
Official URL: http://dx.doi.org/10.1007/s00705-012-1483-8
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We thank T. Swami Krishna, D. Pandary, Md. Yousuf, and C. Lakshminarayana for excellent technical assistance during this study. SCR would like to thank the Jawaharlal Nehru Technological University, Hyderabad, for providing an opportunity to register for the Ph.D. program.
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