Genetic Diversity in Selected Indian Mungbean [Vigna radiata (L.) Wilczek] Cultivars Using RAPD Markers

    Datta, S and Gangwar, S and Kumar, Shiv and Gupta, S and Rai, R and Kaashyap, M and Singh, P and Chaturvedi, S K and Singh, B B and Nadarajan, N (2012) Genetic Diversity in Selected Indian Mungbean [Vigna radiata (L.) Wilczek] Cultivars Using RAPD Markers. American Journal of Plant Sciences, 3 (8). pp. 1085-1091. ISSN 2158-2750

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    Abstract

    Random amplified polymorphic DNA (RAPD) markers were used to study the DNA polymorphism in Indian mungbean cultivars. A total of 60 random primers were used in the study and 33 of them generated reproducible RAPD patterns. Amplification of genomic DNA of most popular 24 Indian mungbean cultivars with these RAPD primers yielded 249 fragments that could be scored, of which 224 were polymorphic, with an average of 7.0 polymorphic fragments per primer. Number of amplified fragments with random primers ranged from 2 (OPI 9) to 17 (OPD 7). Percentage polymorphism ranged from 33% (OPX 5) to a maximum of 100% (OPX 4, OPX 6, OPX 13, OPX 15, OPX 19, OPD 5, OPD 7, OPD 20, OPI 4, OPI 6, OPI 13, OPI 14, OPI 18 and OPF 1), with an average of 90%. The Jaccard’s similarity indices based on RAPD profiles were subjected to UPGMA cluster analysis. And genotypes grouped in two major groups. Sixteen out of 24 released cultivars grouped to cluster I. This indicated the narrow genetic base in the Indian mungbean cultivars used in the study. The details of diversity analysis and possible reasons for narrow genetic base in mungbean cultivars are discussed in the present study.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Fingerprinting; Genetic Diversity; Mungbean; RAPD
    Subjects: Others > Genetics and Genomics
    Others > Agriculture-Farming, Production, Technology, Economics
    Depositing User: Mr Sanat Kumar Behera
    Date Deposited: 04 Sep 2012 03:22
    Last Modified: 08 Oct 2012 12:03
    URI: http://oar.icrisat.org/id/eprint/6109
    Official URL: http://dx.doi.org/10.4236/ajps.2012.38130
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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