Whole-genome scanning for mapping determinacy in Pigeonpea (Cajanus spp.)

Mir, R R and Saxena, R K and Saxena, K B and Upadhyaya, H D and Kilian, A and Cook, D R and Varshney, R K (2013) Whole-genome scanning for mapping determinacy in Pigeonpea (Cajanus spp.). Plant Breeding, 132 (5). pp. 472-478. ISSN 1439-0523

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Abstract

Determinacy is an agronomically important trait in several crop species including pigeonpea. With an objective to investigate determinacy in pigeonpea, a set of 94 pigeonpea lines including 11 determinate (DT) and 83 indeterminate (IDT) lines were used for genotyping with DArT arrays (with 6144 features) and 768 SNP markers using GoldenGate assay. The polymorphism information content (PIC) for these markers varied from 0.02 to 0.50. Association analysis on marker genotyping and phenotyping data showed a significant association (P ≤ 0.01) of determinacy with 19 SNP and 6 DArT markers explaining 8.05–8.58% and 7.26–14.53% phenotypic variation, respectively. Clustering based on entire DArT and SNP markers could not discriminate DT lines from IDT lines; however, analysis with associated markers discriminated DT lines from the IDT lines. Marker–trait associations after validation may prove useful in marker-assisted selection (MAS) involving the development of ideal DT genotypes for environments with moderate growth, tolerance to drought and water logging. This is the first report on mapping of determinacy trait as well as the first report on association mapping for any trait in pigeonpea.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Pigeonpea, determinacy, whole-genome scanning, diversity array technology arrays, GoldenGate SNP assays
Subjects: Others > Genetics and Genomics
Depositing User: Mr Siva Shankar
Date Deposited: 31 Aug 2012 06:52
Last Modified: 26 Jan 2014 10:10
URI: http://oar.icrisat.org/id/eprint/6103
Official URL: http://dx.doi.org/10.1111/j.1439-0523.2012.02009.x
Projects: CGIAR Generation Challenge Programme (GCP), Centre of Excellence (CoE)
Funders: Government of India - Department of Biotechnology
Acknowledgement: UNSPECIFIED
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