An intra-specific consensus genetic map of pigeonpea [Cajanus cajan (L.) Millspaugh] derived from six mapping populations

Bohra, A and Saxena, R K and Gnanesh, B N and Saxena, K B and Byregowda, M and Rathore, A and Kavi Kishor, P B and Cook, D R and Varshney, R K (2012) An intra-specific consensus genetic map of pigeonpea [Cajanus cajan (L.) Millspaugh] derived from six mapping populations. TAG Theoretical and Applied Genetics, 125 (6). pp. 1325-1338. ISSN 1432-2242

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Pigeonpea (Cajanus cajan L.) is an important food legume crop of rainfed agriculture. Owing to exposure of the crop to a number of biotic and abiotic stresses, the crop productivity has remained stagnant for almost last five decades at ca. 750 kg/ha. The availability of a cytoplasmic male sterility (CMS) system has facilitated the development and release of hybrids which are expected to enhance the productivity of pigeonpea. Recent advances in genomics and molecular breeding such as marker-assisted selection (MAS) offer the possibility to accelerate hybrid breeding. Molecular markers and genetic maps are pre-requisites for deploying MAS in breeding. However, in the case of pigeonpea, only one inter- and two intra-specific genetic maps are available so far. Here, four new intra-specific genetic maps comprising 59–140 simple sequence repeat (SSR) loci with map lengths ranging from 586.9 to 881.6 cM have been constructed. Using these four genetic maps together with two recently published intra-specific genetic maps, a consensus map was constructed, comprising of 339 SSR loci spanning a distance of 1,059 cM. Furthermore, quantitative trait loci (QTL) analysis for fertility restoration (Rf) conducted in three mapping populations identified four major QTLs explaining phenotypic variances up to 24 %. To the best of our knowledge, this is the first report on construction of a consensus genetic map in pigeonpea and on the identification of QTLs for fertility restoration. The developed consensus genetic map should serve as a reference for developing new genetic maps as well as correlating with the physical map in pigeonpea to be developed in near future. The availability of more informative markers in the bins harbouring QTLs for sterility mosaic disease (SMD) and Rf will facilitate the selection of the most suitable markers for genetic analysis and molecular breeding applications in pigeonpea.

Item Type: Article
Subjects: Mandate crops > Pigeonpea
Others > Genetics and Genomics
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 12 Jul 2012 04:40
Last Modified: 08 May 2018 07:59
Official URL:
Funders: Indian Council of Agricultural Research , Generation Challenge Programme
Acknowledgement: The authors are thankful to Indian Council of Agricultural Research (ICAR) and Generation Challenge Programme (GCP) of CGIAR for supporting this research. Thanks are also due to Trushar Shah for his suggestions on CMap analysis, Naresh Kumar for generating some genotyping data for ICPB 2049 × ICPL 99050 population published in an earlier article and to Abdul Gafoor, S Ramesh and Ms. G Srivani for their excellent technical support. Open Access
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