Double Haploids in Pearl Millet: Major Project Report Submitted in Partial Fulfillment of the Requirement for the Degree Bachelor of Technology

Niharika, T (2012) Double Haploids in Pearl Millet: Major Project Report Submitted in Partial Fulfillment of the Requirement for the Degree Bachelor of Technology. Other thesis, Joginpally B.R Engineering College.

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Supervisors

SupervisorsOrcid
Mallikarjuna, NaliniICRISAT

Abstract

Anther culture is an important technique to generate double haploid plants. Doubled haploids would help to produce homozygous lines in single step which accelerates the breeding programmes. They are also useful for studying mutagenesis, dosage effects and interaction & linkage of genes. Pearl millet is a diploid (2n=14) plant and summer crop that originated from west Africa. It has become a challenge to develop a reproducible protocol for double haploids in pearl millet. For culture initiation it is necessary to select florets and anthers with appropriate developmental stage of microspore development. The optimal stage for pearl millet anther culture development has been found to be when the microspores are uninucleate with the nucleus in the center and when the spike is still enclosed in the flag leaf sheath. The growth of the donor plants and developmental stage of microspores have been important factors in the intiation of anther cultures. The present study aims to assess the effect of different factors such as growth hormones, media, pretreatments on the induction of androgenesis (division of microspores) and to develop a suitable protocol for producing embryoids of pearl millet in vitro using floret and anther culture.

Item Type: Thesis (Other)
Subjects: Mandate crops > Millets
Others > Genetics and Genomics
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 24 May 2012 09:18
Last Modified: 25 Jul 2012 06:44
URI: http://oar.icrisat.org/id/eprint/5933
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