Ribaut, J M and Hu, X and Hoisington, D A (1997) Use of STSs and SSRs as rapid and reliable preselection tools in a marker-assisted selection-backcross scheme. Plant Molecular Biology Reporter, 15 (2). pp. 154-162. ISSN 1572-9818
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Abstract
We describe a new approach for using suitable STS and SSR markers as a powerful molecular tool for screening segregating populations involved in backcross schemes for marker-assisted selection, as a preselection step. Since it can be applied to very large populations, this preselection strategy allows one to increase substantially the pressure of selection at each backcross generation. The technique is fast and reproducible, and can be made even more efficient and costeffective by simultaneous DNA amplification from different primer pairs. In the example illustrated here, three suitable PCR-based markers were used to complete the selection of 300 individuals out of 2300 in less than one month with two people working on the project.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Uncontrolled Keywords: | DNA isolation, maize, MAS, microsatellites, multiplexing, PCR screening, sequence-tagged sites |
| Subjects: | Others > Maize |
| Depositing User: | Mr Siva Shankar |
| Date Deposited: | 19 Mar 2012 12:39 |
| Last Modified: | 19 Mar 2012 12:39 |
| URI: | http://oar.icrisat.org/id/eprint/5763 |
| Official URL: | http://dx.doi.org/10.1007/BF02812266 |
| Projects: | UNSPECIFIED |
| Funders: | Swiss Development Corporation (SDC), United Nations Development Program (UNDP) |
| Acknowledgement: | [NOTE: D. A. Hoisington is presently with ICRISAT (Patancheru)]---We wish to thank E. Perotti for advice and helpful discussion, as well as E. Huerta and E. Magaha for their excellent technical work. |
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