Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos

    Bohorova, N and Zhang, W and Julstrum, P and McLean, S and Luna, M and Brito, R M and Diaz, L and Ramos, M E and Estanol, P and Pacheco, M and Salgado, M and Hoisington, D A (1999) Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos. TAG Theoretical and Applied Genetics, 99 (3-4). pp. 437-444. ISSN 1432-2242

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    Abstract

    To enhance the level of resistance to insects in tropical maize germplasm we have developed techniques to successfully transform elite tropical maize inbred based on the activity of specific cryI proteins against four major maize pests – corn earworm, fall armyworm, southwestern corn borer and sugarcane borer. Constructs containing cryIAb or cryIAc synthetic genes were used. To generate transgenic plants we have established methods for biolistic bombardment and the selection and regeneration of immature embryos and calli from the elite tropical lines CML72, CML216, CML323, CML327 and hybrids. Transgenic plants resistant to the herbicide BastaTM contained the bands for the cry, bar and gus genes as detected by Southern blot analyses. A simple leaf bioassay presented varying levels of resistance to Southwestern corn borer of transgenic tropical maize carrying the cryIAc gene. Analyses of the progenies confirmed the sexual transmission of the introduced genes and their stable expression

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Uncontrolled Keywords: Tropical maize · Transformation · Plant regeneration · Insect resistance · Bacillus thuringiensis (Bt)
    Subjects: Others > Agriculture-Farming, Production, Technology, Economics
    Others > Maize
    Depositing User: Mr Sanat Kumar Behera
    Date Deposited: 16 Mar 2012 06:55
    Last Modified: 16 Mar 2012 06:55
    URI: http://oar.icrisat.org/id/eprint/5743
    Official URL: http://dx.doi.org/10.1007/s001220051255
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: D. A. Hoisington is presently with ICRISAT (Patancheru)
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