Pellegrineschi, A and et al, . (2004) Stress-induced expression in wheat of the Arabidopsis thaliana DREB1A gene delays water stress symptoms under greenhouse conditions. Genome, 47 (3). pp. 493-500. ISSN 1480-3321
|
PDF
- Published Version
Download (517kB) | Preview |
Abstract
One of the major environmental factors limiting plant productivity is lack of water. This is especially true for the major cereals maize, rice, and wheat, which demonstrate a range of susceptibility to moisture deficit. Although conventional breeding and marker-assisted selection are being used to develop varieties more tolerant to water stress, these methods are time and resource consuming and germplasm dependent. Genetic engineering is attractive because of its potential to improve abiotic stress tolerance more rapidly. Transcription factors have been shown to produce multiple phenotypic alterations, many of which are involved in stress responses. DREB1A, a transcription factor that recognizes dehydration response elements, has been shown in Arabidopsis thaliana to play a crucial role in promoting the expression of drought-tolerance genes. In our efforts to enhance drought tolerance in wheat, the A. thaliana DREB1A gene was placed under control of a stress-inducible promoter from the rd29A gene and transferred via biolistic transformation into bread wheat. Plants expressing the DREB1A gene demonstrated substantial resistance to water stress in comparison with checks under experimental greenhouse conditions, manifested by a 10-day delay in wilting when water was withheld.Key words: Wheat transformation, MPB Bobwhite 26, DREB1A, rd29 promoter, moisture stress tolerance
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Others > Genetics and Genomics Others > Wheat |
| Depositing User: | Mr Sanat Kumar Behera |
| Date Deposited: | 15 Mar 2012 08:28 |
| Last Modified: | 15 Mar 2012 08:36 |
| URI: | http://oar.icrisat.org/id/eprint/5726 |
| Official URL: | http://dx.doi.org/10.1139/g03-140 |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | D. A. Hoisington is presently with ICRISAT (Patancheru) |
| Links: |
Actions (login required)
 |
View Item |
Altmetric
Altmetric