A Disease of Pearl Millet in Zimbabwe Caused by Pantoea agglomerans

Frederickson, D E and Monyo, E S and King, S B and Odvody, G N (1997) A Disease of Pearl Millet in Zimbabwe Caused by Pantoea agglomerans. Plant Disease, 81 (8). p. 959. ISSN 0191-2917

PDF - Published Version
Download (17kB) | Preview


Necrosis at the leaf tips and margins of pearl millet (Pennisetum glaucum (L.) R. Br.) was observed in 1995 in a Pseudomonas syringae resistance screening nursery near Bulawayo, Zimbabwe. Straw-colored lesions with a chlorotic edge often extended the leaf length, and were atypical of the round spots, with a brown margin, caused by P. syringae (1). Bacteria were isolated from cut lesions macerated in water by dilution streaking onto King's medium B and nutrient agar. A gram-negative, nonfluorescent, fermentative, rod-shaped bacterium, forming yellow colonies on nutrient agar was consistently observed. Three pots of 10, 2-to 3-week-old seedlings of a susceptible cultivar, 852B, were inoculated with a 108 CFU per ml suspension from cultures by misting or injection into the whorl. In three experiments, the treatment and uninoculated control were incubated at 25°C and 95% relative humidity for 48 h before transfer to the greenhouse. The original symptoms of watersoaking at leaf tips and margins were observed after 4 days. Necrotic lesions surrounded by chlorotic tissue were observed a day later. Fluorescence on King's medium B, and levan, oxidase, potato-rot, arginine dihydrolase, 2-keto gluconate, nitrate reduction, gelatin, phenylalanine deaminase, and acid from starch tests were negative. Tobacco hypersensitivity, acid from sucrose and glycerol, aesculin hydrolysis, lipase, indole production, and growth on tetrazolium chloride were positive. The identification of the pathogen to the species level as Pantoea agglomerans (Ewing and Fife 1972) Gavini et al. 1989, formerly Erwinia herbicola, was by fatty acid analysis by the International Mycological Institute (Egham, Surrey, UK). P. agglomerans was recorded as a pathogen of pearl millet in India in 1958 (2).

Item Type: Article
Subjects: Mandate crops > Millets
Others > Entomology
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 09 Mar 2012 05:33
Last Modified: 09 Mar 2012 05:33
URI: http://oar.icrisat.org/id/eprint/5697
Official URL: http://dx.doi.org/10.1094/PDIS.1997.81.8.959D
Acknowledgement: UNSPECIFIED
View Statistics

Actions (login required)

View Item View Item