Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum

Gopalakrishnan, S and Beale, M H and Ward, J L and Strange, R N (2005) Chickpea wilt: identification and toxicity of 8-O-methyl-fusarubin from Fusarium acutatum. Phytochemistry, 66 (13). pp. 1536-1539. ISSN 0031-9422

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Abstract

Fusarium acutatum was isolated from wilting chickpea plants in Pakistan. Filtrates from cultures grown on a defined liquid medium caused permanent wilting of chickpea cuttings and killed cells, isolated enzymically from healthy plants, in a bioassay. Toxic activity was retained by a cyano solid phase extraction cartridge and the toxin was isolated by elution from the cartridge in acetonitrile and Si-gel thin layer chromatography of the eluate. Analytical HPLC of the compound on a cyano column with diode array detection gave a single peak with a homogeneous spectrum and λmax at 224 and 281 nm. NMR and mass spectral studies showed that the toxin was 8-O-methyl-fusarubin. The pure compound caused permanent wilting of chickpea cuttings and the LD50 value in the cell bioassay was 327 ng/ml.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Chickpea
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	18 Feb 2012 09:34
Last Modified:	18 Feb 2012 09:34
URI:	http://oar.icrisat.org/id/eprint/5541
Official URL:	http://dx.doi.org/10.1016/j.phytochem.2005.04.037
Projects:	UNSPECIFIED
Funders:	European Union
Acknowledgement:	We thank Mr. Muhammad Hanif for making the isolations of fungi and the European Union for support with the grant “Ascorab” (Contract No. ICA4-CT-2000-30003). We thank Jason Ejimadu (RRES) for mass spectrometry
Links:	Author

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