Physiological and biochemical characteristics of fast growing rhizobia of Sesbania bispinosa in the in-vitro condition

Gopalakrishnan, S and Girish, A G (1999) Physiological and biochemical characteristics of fast growing rhizobia of Sesbania bispinosa in the in-vitro condition. Legume Research, 22 (4). pp. 227-232. ISSN 0250-5371

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Abstract

Five different strains of rhizobia were isolated from root nodules of Sesbania bispinosa. The native isolates varied in symbiotic performance in terms of nodule fresh weight and plant dry weight. The fast growing rhizobial isolates produced high nitrogenase activity, high nodulation count and biomass. Characterization of these different strains with regard to explanta nitrogenase, possession of intrinsic antibiotic resistance, ability to utilize various carbon sources, temperature tolerance and salinity tolerance were done after nodulation. All the strains exhibited explanta nitrogenase activity and exhibited ampicillin, chloramphenicol, kanamycin, penicillin and nalidixic acid resistance and sensitive to gentamycin and tetracycline. The isolates could utilize wide range of carbon sources like mannitol, sucrose, dextrose, galactose, maltose, lactose, and cellobiose except citrate. These strains could grow at wide range of temperatures up to 44°C, and salinity levels of 600 mM NaCl

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Others
Depositing User:	Ms K Syamalamba
Date Deposited:	14 Feb 2012 03:38
Last Modified:	18 Mar 2013 06:01
URI:	http://oar.icrisat.org/id/eprint/5455
Official URL:
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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