Genetic structure and diversity of wild sorghum populations (Sorghum spp.) from different eco-geographical regions of Kenya

    Muraya, M M and de Villiers, S and Parzies, H K and Mutegi, E and Sagnard, F and Kanyenji, B M and Kiambi, D and Geiger, H H (2011) Genetic structure and diversity of wild sorghum populations (Sorghum spp.) from different eco-geographical regions of Kenya. TAG Theoretical and Applied Genetics, 123 (4). pp. 571-583. ISSN 1432-2242

    [img] PDF - Published Version
    Restricted to ICRISAT users only
    Download (690kB) | Request a copy

    Abstract

    Wild sorghums are extremely diverse phenotypically, genetically and geographically. However, there is an apparent lack of knowledge on the genetic structure and diversity of wild sorghum populations within and between various eco-geographical regions. This is a major obstacle to both their effective conservation and potential use in breeding programs. The objective of this study was to assess the genetic diversity and structure of wild sorghum populations across a range of eco-geographical conditions in Kenya. Sixty-two wild sorghum populations collected from the 4 main sorghum growing regions in Kenya were genotyped using 18 simple sequence repeat markers. The study showed that wild sorghum is highly variable with the Coast region displaying the highest diversity. Analysis of molecular variance showed a significant variance component within and among wild sorghum populations within regions. The genetic structure of wild sorghum populations indicated that gene flow is not restricted to populations within the same geographic region. A weak regional differentiation was found among populations, reflecting human intervention in shaping wild sorghum genetic structure through seed-mediated gene flow. The sympatric occurrence of wild and cultivated sorghums coupled with extensive seed-mediated gene flow, suggests a potential crop-to-wild gene flow and vice versa across the regions. Wild sorghum displayed a mixed mating system. The wide range of estimated outcrossing rates indicate that some environmental conditions may exist where self-fertilisation is favoured while others cross-pollination is more advantageous.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Sorghum
    Depositing User: Users 6 not found.
    Date Deposited: 04 Jan 2012 03:36
    Last Modified: 04 Jan 2012 03:36
    URI: http://oar.icrisat.org/id/eprint/5240
    Official URL: http://dx.doi.org/10.1007/s00122-011-1608-6
    Projects: UNSPECIFIED
    Funders: United States Agency for International Development, Biotechnology and Biodversity Interface Program
    Acknowledgement: This study was funded by the United States Agency for International Development (USAID) Biotechnology and Biodversity Interface Program (BBI; Dr. Fabrice Sagnard), the Institute of plant Breeding and Population Genetics at the University of Hohenheim, Germany, and German Academic Exchange Service (DAAD: A0523923). USAID-BBI funded field experiments and collection trips. Costs of laboratory infrastructure and consumables at the Institute of plant Breeding and Population Genetics at the University of Hohenheim, Germany, were shared by BBI and the University of Hohenheim. We acknowledge the Kenya Agricultural Research Institute and Ben Kanyenji who supervised the collection of genetic materials in full compliance with regulations according to the Convention on Biological Diversity (CBD).
    Links:
    View Statistics

    Actions (login required)

    View Item View Item
    OAR@ICRISAT is powered by EPrints 3 which is developed by the School of Electronics and Computer Science at the University of Southampton. More information and software credits.