Impact of low lignin containing brown midrib sorghum mutants to harness biohydrogen production using mixed anaerobic consortia

Prakasham, R. S. and Brahmaiah, P. and Nagaiah, D. and Srinivasa Rao, P and Reddy, B V S and Sreenivas Rao, R. and Hobbs, P. J. (2012) Impact of low lignin containing brown midrib sorghum mutants to harness biohydrogen production using mixed anaerobic consortia. International Journal of Hydrogen Energy, 37 (4). pp. 3186-3190. ISSN 0360-3199

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Abstract

Three low lignin containing bmr 3 derivatives, namely DRT 07K1, DRT 07K6 and DRT 07K15 developed through backcrossing were used along with the parent, bmr 3 source mutant (IS 21888) for evaluation of biohydrogen production. Results demonstrated that biohydrogen production varied amongst bmr derivatives under similar fermentation conditions. Significant negative correlation was observed between lignin content and fermentative biohydrogen production. All bmr derivatives with lower lignin content produced higher levels of biohydrogen compared to source bmr 3 (IS 21888) which has more lignin content. The maximum and a minimum biohydrogen production observed was 72 and 50 ml/g Total Volatile Solids (TVS) for the DRT 07K6 bmr3 derivative and bmr 3 (IS 21888) respectively. Acetate and butyrate were accounted >85% of volatile fatty acids, indicating acid type fermentations. Statistical analysis revealed that all bmr mutant derivatives with respect to source differ significantly in cumulative biohydrogen production, plant height, grain yield and lignin content. Biohydrogen production from biomass associated at least two different levels, one at lignin entanglement another at the polymeric nature of cellulose and hemicellulose. Further studies are necessary to determine the effect of biomass structure associated with different bmr traits on the microbial growth and biohydrogen production rate

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Anaerobic fermentation, Biohydrogen, Brown midrib mutants Microbial consortia, Sorghum stalks
Subjects: Mandate crops > Sorghum
Depositing User: Mr Siva Shankar
Date Deposited: 26 Dec 2011 07:44
Last Modified: 29 Dec 2016 08:41
URI: http://oar.icrisat.org/id/eprint/5156
Official URL: http://dx.doi.org/10.1016/j.ijhydene.2011.11.082
Projects: BBSRC-INDIA
Funders: Council of Scientific and Industrial Research(India)
Acknowledgement: The authors are grateful to Director of Indian Institute of Chemical Technology, Hyderabad, India and European commission funded SWEETFUEL project for the valuable support. P Brahmaiah, and D Nagaiah acknowledge CSIR for financial support as Senior Research Fellowship. This work is partially supported by BBSRC-INDIA partnering award. All authors acknowledge for Dr Michael Blummel, International Livestock Research Institute (ILRI), Patancheru for analyzing lignin and ME in bmr derivatives
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