Nolt, B L and Rajeshwari, R and Bharathan, N and Reddy, D V R (1983) Improved Serological Techniques for the Detection and Identification of Groundnut Viruses. In: Proceedings of the National Seminar on Management of Diseases of Oilseed Crops, 21st - 22nd January 1983, Madurai.
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Abstract
Serology is indispensable for the detection and identification of plant viruses. Recently, the highly sensitive enzyme-linked immunosorbent assay (ELISA) and immunosorbent electron microscopy (ISEM) techniques have been developed and may replace some of the conventional serological methods. The direct or standard double-antibody sandwich (DAS) form of ELISA. as first described by Clark and Adams (1977), has wide applications in plant virology. An indirect form of ELISA (I-ELISA) has recently been developed by Barbara and Clark (1982). The ISEM procedure developed by Derrick (1973) combines the specificity of serology with coventional electron microscopy. DAS-ELISA, I-ELISA and ISEM have been adapted for the detection and identification of several groundnut viruses. This paper will provide a description of each technique and its application for the detection and characterization of viruses occurring on groundnut in India.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Mandate crops > Groundnut |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 28 Dec 2011 03:18 |
| Last Modified: | 28 Dec 2011 03:18 |
| URI: | http://oar.icrisat.org/id/eprint/5142 |
| Acknowledgement: | UNSPECIFIED |
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