Antagonistic activity of bacteria inhabiting composts against soil-borne plant pathogenic fungi

Hameeda, B and Rupela, O P and Reddy, G (2006) Antagonistic activity of bacteria inhabiting composts against soil-borne plant pathogenic fungi. Indian Journal of Microbiology, 46 (4). pp. 389-396. ISSN 0046-8991

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A total of 207 bacteria were isolated from 4 different sources, i.e. farm waste compost (FWC), rice straw compost (RSC), Gliricidia vermicompost (GVC) and macrofauna (earthworms, centipedes, slugs and snails). All the isolates were tested for antagonistic activity on 4 soil-borne plant pathogenic fungi, i.e. Sclerotium rolfsii, Macrophomina phaseolina, Fusarium solani and Fusarium oxysporum. Percentage of antagonistic bacteria varied from 19 in FWC to 39 in GVC. Of the 207 isolates, 18 were antagonistic to all 4 soil-borne plant pathogenic fungi. Inhibition was maximum on Kings B medium (63%), followed by glycerol casamino yeast extract (GCY; 49%) and potato dextrose agar (PDA; 46%). Identification of the 18 antagonistic isolates revealed that 12 belonged to the genus Bacillus and 6 were Pseudomonas. B. licheniformis (EB 13) and Pseudomonas sp. (CDB 35) showed antifungal effect higher than other bacteria in plate culture conditions. These strains also showed significant reduction in fungal biomass when grown in broth culture. PDA amended with zinc sulfate further improved antifungal activity of EB 13 and CDB 35. In glasshouse conditions, soil amended with glucose and/or zinc in combination with EB 13 or CDB 35 reduced M. phaseolina infection in sorghum roots and improved shoot weight

Item Type: Article
Uncontrolled Keywords: Antagonistic bacteria, composts, pathogenic fungi, soil borne, sorghum
Subjects: Mandate crops > Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 05 Dec 2011 08:12
Last Modified: 05 Dec 2011 08:12
Official URL:
Acknowledgement: The authors thank Dr RP Thakur of ICRISAT for providing pathogenic cultures of fungi. Doctoral fellowship to Ms Hameeda Bee by Jawaharlal Nehru Memorial Fund, New Delhi is gratefully acknowledged
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