Histological aspects of in vitro root and shoot differentiation from cotyledon explants of Brassica juncea (L.) czern

Sharma, K K and Bhojwani, S S (1990) Histological aspects of in vitro root and shoot differentiation from cotyledon explants of Brassica juncea (L.) czern. Plant Science, 69 (2). pp. 207-214. ISSN 0168-9452

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Abstract

Under fairly simple culture conditions, excised cotyledons of Brassica juncea undergo high frequencies of either root formation on growth regulator-free Murashige and Skoog (MS) medium or shoot formation on MS + 5.0 μM N6-benzyladenine (BA). Temporal histological events of organ differentiation processes revealed that while root formation was endogenous in origin and did not involve callusing, shoot bud differentiation occurred after the formation of meristematic nodules at the cut end. While root primordia differentiated from the petiolar cut end after 4 days in culture, the formation of a meristematic nodule at the same end occurred by 4–6 days which further lead to the differentiation of shoot meristem by 8–10 days. Although the initial accumulation of starch in organogenic cotyledons was the same during both root and shoot formation, its depletion was faster in shoot forming cultures suggesting that this process is more energy requiring than rhizogenesis.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Brassica juncea; cotyledon culture; histology; organogenesis; regeneration; tissue culture
Subjects:	Others > Plant Virology Others > Agriculture-Farming, Production, Technology, Economics
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	03 Dec 2011 12:25
Last Modified:	03 Dec 2011 12:25
URI:	http://oar.icrisat.org/id/eprint/4777
Official URL:	http://dx.doi.org/10.1016/0168-9452(90)90119-9
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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