A method for measuring the transfer of fixed nitrogen from free-living bacteria to higher plants using 15N2

Giller, K E and Day, J M and Dart, P J and Wani, S P (1984) A method for measuring the transfer of fixed nitrogen from free-living bacteria to higher plants using 15N2. Journal of Microbiological Methods, 2 (6). pp. 307-316. ISSN 0167-7012

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Abstract

An apparatus is described in which the root systems of up to ten plants can be simultaneously incubated with 15N2 gas mixtures, while the aerial environment remains undisturbed. The size of incubation chamber and the number of chambers can be adjusted depending on the size and growth stage of the plants being tested. The apparatus has the facility for the recovery of gas mixtures, sampling and adjustment of gas concentrations, and control of soil moisture status within a sealed system. Possible experimental applications are suggested and some preliminary results presented which demonstrate bacterial nitrogen fixation and uptake into Sorghum seedlings.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Cereals, Grasses, Nitrogen-15 fixation, Nitrogen fixation (assoclative), Sorghum
Subjects: Mandate crops > Sorghum
Others > Fertilizer Applications
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 01 Dec 2011 12:51
Last Modified: 02 Jan 2012 09:11
URI: http://oar.icrisat.org/id/eprint/4643
Official URL: http://dx.doi.org/10.1016/0167-7012(84)90049-6
Projects: UNSPECIFIED
Funders: Overseas Development Administration
Acknowledgement: We thank Angela Davitt and M. Babu Rao for technical assistance and Dr. J.F. Witty for helpful discussions. This work was funded through Research Scheme R3648 of the Overseas Development Administration
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