Anjaiah, V and Mehan, V K and Jayanthi, S and Reddy, D V R and McDonald, D (1989) Enzyme-Linked Immunosorbent Assay (ELISA) for Aflatoxin B1 Estimation in Groundnuts. In: International Workshop on Aflatoxin Contamination of Groundnut, 6-9 Oct 1987, Patancheru, Andhra Pradesh, India.
![[img]](http://oar.icrisat.org/style/images/fileicons/application_pdf.png)
|
PDF
- Published Version
Download (1MB) | Preview |
Abstract
The commercially available hapten, afla B1-oxime-bovine serum albumin, was used to produce an antiserum in rabbits. The same hapten was coupled with alkaline phosphatase (hapten-BSA-ALP) and used in the competitive direct enzyme-linked immunosorbent assay (ELISA) for the detection of aflatoxin B1. Aflatoxin B1 was extracted in methanol from naturally contaminated or 'spiked' groundnut seed samples. Wells of a polystyrene microtitre plate were coated with the antiserum, the plates were washed in PBS-Tween, aflatoxin B1 standards or groundnut sample extracts, and hapten-BSA-ALP conjugate were added and the plates incubated. The plates were again washed, and the amount of conjugate bound to the antibody was determined after addition of the substrate, p-nitro-phenylphosphate. The hapten-BSA-ALP conjugate has advantages in stability, simplicity of preparation, and high specificity, over the conventional toxin-enzyme conjugate in direct competitive ELISA. The assay method is more rapid and less expensive than the physico-chemical methods of aflatoxin analysis and it can detect levels of aflatoxin B1 as low as 50 picograms.
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Mandate crops > Groundnut |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 04 Dec 2011 12:00 |
| Last Modified: | 04 Dec 2011 12:00 |
| URI: | http://oar.icrisat.org/id/eprint/4481 |
| Acknowledgement: | UNSPECIFIED |
| Links: |
Actions (login required)
 |
View Item |
Altmetric
Altmetric