Mallikarjuna, N and Sastri, D C (1985) In vitro Culture of Ovules and Embryos from some Incompatible Interspecific Crosses in the Genus Arachis L. In: Proceedings of an International Workshop on Cytogenetics of Arachis, 31 Oct - 2 Nov 1983, Patancheru, Andhra Pradesh, India.
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Abstract
In vrtro culture of ovules and embyros IS commonly used to produce hybrrds from rncompalrble crosses. In several mlerspecrlrc crossar in Arachlr, ovule and or embryo cullure rs necessary because the ovules do nor develop lully in vrvo Ovule srzes from drfferml crosses and dliierenr hormone lrealmenrs range lrom less then 0 3 mm lo 5 0 mm, rarely 7 0 mm Ovules smaller than 3 0 mm had to be cultured mlrre as the proembryos rhey conlamed were roo small lor drsseclron and cullure Ovules larger rhan 3 Omm were drssecred and lherr embryos cullured All the ovules were culturedon MS medra with or wrlhoul agar and with diilerenl concenlrrtrons of various hormones Dependrng on the hormones used and therr concenlratrons, drilerenr responses were observed Surface callus formatron, greenmg, swellrng. and browntng were common rn cullured ovules Embryos emerged from some of the ovules end planllers have been oblarned Cultured embryos showed srmrler responses, and some cultured embryos germinated, and developed rnlo planllsls
| Item Type: | Conference or Workshop Item (Paper) |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Mandate crops > Groundnut |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 06 Dec 2011 03:30 |
| Last Modified: | 06 Dec 2011 03:30 |
| URI: | http://oar.icrisat.org/id/eprint/4159 |
| Acknowledgement: | UNSPECIFIED |
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