Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers

Varshney, R K and Chen, W and Li, Y and Bharti, A K and Saxena, R K and Schlueter, J A and Donoghue, M T A and Azam, S and Fan, G and Whaley, A M and Farmer, A D and Sheridan, J and Iwata, A and Tuteja, R and Penmetsa, R V and Wu, W and Upadhyaya, H D and Yang, S P and Shah, T and Saxena, K B and Michael, T and McCombie, W R and Yang, B and Zhang, G and Yang, H and Wang, J and Spillane, C and Cook, D R and May, G D and Xu, X and Jackson, S A (2012) Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers. Nature Biotechnology, 30 (1). pp. 83-89. ISSN 1546-1696

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Abstract

Pigeonpea is an important legume food crop grown primarily by smallholder farmers in many semi-arid tropical regions of the world. We used the Illumina next-generation sequencing platform to generate 237.2 Gb of sequence, which along with Sanger-based bacterial artificial chromosome end sequences and a genetic map, we assembled into scaffolds representing 72.7% (605.78 Mb) of the 833.07 Mb pigeonpea genome. Genome analysis predicted 48,680 genes for pigeonpea and also showed the potential role that certain gene families, for example, drought tolerance–related genes, have played throughout the domestication of pigeonpea and the evolution of its ancestors. Although we found a few segmental duplication events, we did not observe the recent genome-wide duplication events observed in soybean. This reference genome sequence will facilitate the identification of the genetic basis of agronomically important traits, and accelerate the development of improved pigeonpea varieties that could improve food security in many developing countries.

Item Type: Article
Divisions: UNSPECIFIED
CRP: UNSPECIFIED
Uncontrolled Keywords: Draft genome sequence, pigeonpea
Subjects: Mandate crops > Pigeonpea
Depositing User: Mr Sanat Kumar Behera
Date Deposited: 11 Nov 2011 04:43
Last Modified: 08 Aug 2018 05:49
URI: http://oar.icrisat.org/id/eprint/3886
Official URL: http://dx.doi.org/10.1038/nbt.2022
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: UNSPECIFIED
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