Evaluation of actinomycete isolates obtained from herbal vermicompost for the biological control of Fusarium wilt of chickpea

Gopalakrishnan, S and Pande, S and Sharma, M and Humayun, P and Kiran, B K and Sandeep, D and Sree Vidya, M and Deepthi, K and Rupela, Om (2011) Evaluation of actinomycete isolates obtained from herbal vermicompost for the biological control of Fusarium wilt of chickpea. Crop Protection, 30 (8). pp. 1070-1078. ISSN 0261-2194

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A total of 137 actinomycetes cultures, isolated from 25 different herbal vermicomposts, were characterized for their antagonistic potential against Fusarium oxysporum f. sp. ciceri (FOC) by dual-culture assay. Of the isolates, five most promising FOC antagonistic isolates (CAI-24, CAI-121, CAI-127, KAI-32 and KAI-90) were characterized for the production of siderophore, cellulase, protease, hydrocyanic acid (HCN), indole acetic acid (IAA) and antagonistic potential against Rhizoctonia bataticola, which causes dry root rot in chickpea (three strains viz. RB-6, RB-24 and RB-115) and sorghum (one strain). All of the five FOC antagonistic isolates produced siderophore and HCN, four of them (except KAI-90) produced IAA, KAI-32 and KAI-90 produced cellulase and CAI-24 and CAI-127 produced protease. In the dual-culture assay, three of the isolates, CAI-24, KAI-32 and KAI-90, also inhibited all three strains of R. bataticola in chickpea, while two of them (KAI-32 and KAI-90) inhibited the tested strain in sorghum. When the FOC antagonistic isolates were evaluated further for their antagonistic potential in the greenhouse and wilt-sick field conditions on chickpea, 45–76% and 4–19% reduction of disease incidence were observed, respectively compared to the control. The sequences of 16S rDNA gene of the isolates CAI-24, CAI-121, CAI-127, KAI-32 and KAI-90 were matched with Streptomyces tsusimaensis, Streptomyces caviscabies, Streptomyces setonii, Streptomyces africanus and an identified species of Streptomyces, respectively using the BLAST searching. This study indicated that the selected actinomycete isolates have the potential for biological control of Fusarium wilt disease in chickpea.

Item Type: Article
Uncontrolled Keywords: Antagonistic actinomycetes; Biocontrol; Chickpea; Wilt; Fusarium oxysporum f. sp. ciceri; Streptomyces spp.
Agro Tags: <b>Agrotags</b> - bacteria | planting | isolation | fungi | biochemical compounds | wilts | soil | chickpeas | diseases | application methods <br><b>Fishtags</b> - drying <br><b>Geopoliticaltags</b> - act | india | kenya
Subjects: Mandate crops > Chickpea
Depositing User: Siva Shankar
Date Deposited: 26 May 2011 10:21
Last Modified: 16 Sep 2013 09:06
URI: http://oar.icrisat.org/id/eprint/38
Official URL: http://dx.doi.org/10.1016/j.cropro.2011.03.006
Funders: National Bureau of Agriculturally Important Microorganisms
Acknowledgement: We thank the National Bureau of Agriculturally Important Microorganisms for providing financial support. We also thank all the staff of biocontrol unit of ICRISAT including M/s PVS Prasad, P Manohar, B Nagappa, M Satyam, D Barath, A Jabbar and D Nagabushnam for their significant inputs in the laboratory, greenhouse and field studies
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