An integrated genetic map and a new set of simple sequence repeat markers for pearl millet, Pennisetum glaucum

Qi, X and Pittaway, T S and Lindup, S and Liu, H and Waterman, E and Padi, F K and Hash, C T and Zhu, J and Gale, M D and Devos, K M (2004) An integrated genetic map and a new set of simple sequence repeat markers for pearl millet, Pennisetum glaucum. Theoretical and Applied Genetics, 108 (7). pp. 1485-1493. ISSN 0040-5752

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Abstract

Over the past 10 years, resources have been established for the genetic analysis of pearl millet, Pennisetum glaucum (L.) R. Br., an important staple crop of the semi-arid regions of India and Africa. Among these resources are detailed genetic maps containing both homologous and heterologous restriction fragment length polymorphism (RFLP) markers, and simple sequence repeats (SSRs). Genetic maps produced in four different crosses have been integrated to develop a consensus map of 353 RFLP and 65 SSR markers. Some 85% of the markers are clustered and occupy less than a third of the total map length. This phenomenon is independent of the cross. Our data suggest that extreme localization of recombination toward the chromosome ends, resulting in gaps on the genetic map of 30 cM or more in the distal regions, is typical for pearl millet. The unequal distribution of recombination has consequences for the transfer of genes controlling important agronomic traits from donor to elite pearl millet germplasm. The paper also describes the generation of 44 SSR markers from a (CA)n-enriched small-insert genomic library. Previously, pearl millet SSRs had been generated from BAC clones, and the relative merits of both methodologies are discussed

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Millets
Depositing User:	Ms K Syamalamba
Date Deposited:	07 Nov 2011 10:10
Last Modified:	07 Nov 2011 10:11
URI:	http://oar.icrisat.org/id/eprint/3767
Official URL:	http://dx.doi.org/10.1007/s00122-004-1765-y
Projects:	UNSPECIFIED
Funders:	Biotechnology and Biological Sciences Research Council (BBSRC) through a David Phillips Research Fellowship
Acknowledgement:	This manuscript is largely an output from projects funded by the Plant Science Research Programme (contracts R6951 and R7979) of the UK Department for International Development (DFID) for the benefit of developing countries. The views expressed are not necessarily those of DFID. KMD acknowledges funding from the Biotechnology and Biological Sciences Research Council (BBSRC) through a David Phillips Research Fellowship
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