Effect of Various Stains on The Stability And Particle Length Distribution of Three Poty Viruses

    Manohar, S K and Rajeshwari, R (1985) Effect of Various Stains on The Stability And Particle Length Distribution of Three Poty Viruses. Indian Journal of Virology, 1 (2). pp. 157-162. ISSN 0974-0120

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    Abstract

    Four negative stains were tested on Peanut mottle virus (PMV), two irolater of Peanut green mosaic virus-systemic necrosis (PGMV-SN) ar~dsy etrmic mosaic (PGM V-SM), to datrrmlne their effects on the stability and particle length distribution. Whan phosphotunngstate(PleTA ) at pH 7.0 was used, particle lengh and width of PMV and PGMV-SM werr conside~ably affecrnd rasulting in variation, whereas PGMV-SN war la18 affected. When uranyl acotete (UA) and urrnyl formate (UF) ware ured, tho prrlicler of PGMV-SM and PMV were better preserved, in contrast to PGMV-SN which was unstable in both the uranyl stains. PGMV.SN was well presarved in ammonium molybdrte (AM) o f pHs 3.5 and 6.5 but was d;sruoted in OH 8.0. Although PMV could be stairled with AM of pH 3.6, particles ware poorly stained In wM ot pHs 6.6 and 8.0. Pertlcles ol PGMV-SM were poorly strined in AM of all the three pH valuer.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Others > Plant Virology
    Others > Fertilizer Applications
    Depositing User: Mr Charan Sai Ch
    Date Deposited: 13 Nov 2011 07:58
    Last Modified: 13 Nov 2011 07:58
    URI: http://oar.icrisat.org/id/eprint/3510
    Official URL:
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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