The use of hydrogen peroxide for the digestion and determination of total nitrogen in chickpea (Cicer arietinum L.) and pigeonpea (Cajanus cajan L.)

    Singh, U and Sahrawat, K L and Jambunathan, R and Burford, J R (1984) The use of hydrogen peroxide for the digestion and determination of total nitrogen in chickpea (Cicer arietinum L.) and pigeonpea (Cajanus cajan L.). Journal of the Science of Food and Agriculture, 35 (6). pp. 640-646. ISSN 0022-5142

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    Abstract

    The use of hydrogen peroxide as an alternative to catalysts in the determination of nitrogen in plant materials has been investigated. Nitrogen determination in leaf, stem and seed samples of chickpea and pigeonpea was carried out by three digestion procedures, using hydrogen peroxide (H2O2 digestion) without a catalyst, and with mercury or selenium as catalysts (catalyst digestions). The nitrogen values obtained by the three digestion procedures did not differ significantly from each other when examined by microKjeldahl (MKJ) using mercury as a catalyst, and by a Technicon auto analyser (TAA) using selenium as catalyst. The gradual addition of H2O2, as recommended previously, was found to be unnecessary. In view of the cost and health hazards implicated with the use of mercury and selenium for digestion, the procedure based on H2O2 digestion is preferable for large scale N determinations in plant tissue and seed samples of these pulse crops. The results sugest that the H2O2 digestion technique can be conveniently adapted for total N analysis in plant tissues and grain samples by either TAA or MKJ method.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Chickpea
    Mandate crops > Pigeonpea
    Depositing User: Mr Charan Sai Ch
    Date Deposited: 30 Oct 2011 13:38
    Last Modified: 30 Oct 2011 13:38
    URI: http://oar.icrisat.org/id/eprint/3413
    Official URL: http://dx.doi.org/10.1002/jsfa.2740350609
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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