Factors Influencing Nitrogenase Activity (Acetylene Reduction) by Root Nodules of Groundnut, Arachis hypogaea L.

Nambiar, P T C and Dart, P J (1983) Factors Influencing Nitrogenase Activity (Acetylene Reduction) by Root Nodules of Groundnut, Arachis hypogaea L. Peanut Science, 10 (1). pp. 26-29.

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Abstract

Acetylene reduction assay, used to measure nitrogenase activity of legume root nodules, is influenced by environmental factors, which limit its application. The effects of some of the environmental factors on acetylene reduction by groundnut root nodules are described. The activity was nonlinear during the first hour of incubation. Assay temperature above 25 C decreased the activity. Washing the nodulated roots prior to the assay also decreased the activity. The activity was influenced by light intensity, soil moisture, and moisture content in the incubation bottle. Diurnal fluctuation with one maximum and one minimum activity period during a 24 hour cycle was observed. Nitrogenase activity was higher during the postrainy season compared to that of the rainy season. A virginia cultivar Kadiri-71 had higher nitrogenase activity than a dwarf valencia cultivar, MH 2.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Groundnut
Depositing User:	Mr Charan Sai Ch
Date Deposited:	31 Oct 2011 08:57
Last Modified:	31 Oct 2011 08:57
URI:	http://oar.icrisat.org/id/eprint/3378
Official URL:	http://dx.doi.org/10.3146/i0095-3679-10-1-8
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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