Genetic transformation of pigeonpea with rice chitinase gene

Kumar, S M and Kumar, B K and Sharma, K K and Devi, P (2004) Genetic transformation of pigeonpea with rice chitinase gene. Plant Breeding, 123 (5). pp. 485-489.

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Abstract

With a long-term plan to develop transgenic pigeonpea with resistance to fungal disease, the transfer of a rice chitinase gene to pigeonpea[Cajanus cajan (L.) Millsp.] is reported here. The rice chitinase gene harboured in the plasmid pCAMBIA 1302:RChit was delivered via the Agrobacterium-mediated method to the cotyledonary node explants followed by subsequent regeneration of complete plants on selection media containing hygromycin. Putative transformed pigeonpea plants were recovered with stringent selection pressure and confirmed using molecular techniques. Stable integration and expression of the chitinase gene has been confirmed in the T0 and T1 transgenics through molecular analysis.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Pigeonpea
Depositing User:	Ms Vibha Raju
Date Deposited:	28 Oct 2011 16:06
Last Modified:	28 Oct 2011 16:06
URI:	http://oar.icrisat.org/id/eprint/3300
Official URL:	http://dx.doi.org/10.1111/j.1439-0523.2004.01028.x
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	UNSPECIFIED
Links:	Author

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