RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet

Rajeshwari, R and Sivaramakrishnan, S and Smith, R L and Subrahmanyam, N C (1994) RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet. TAG Theoretical and Applied Genetics, 88 (3-4). pp. 441-448. ISSN 1432-2242

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Abstract

Mitochondrial DNA (mtDNA) from 13 cytoplasmic male-sterile (cms) lines from diverse sources were characterized by Southern blot hybridization to pearl millet and maize mtDNA probes. Hybridization patterns of mtDNA digested with PstI, BamHI, SmaI or XhoI and probed with 13.6-, 10.9-, 9.7- or 4.7-kb pearl millet mtDNA clones revealed similarities among the cms lines 5141 A and ICMA 1 (classified as the S-A1 type of cytoplasm based on fertility restoration patterns), PMC 30A and ICMA 2. The remaining cms lines formed a distinct group, within which three subgroups were evident. Among the maize mitochondiral gene clones used, the coxI probe revealed two distinct groups of cytoplasms similar to the pearl millet mtDNA clones. The atp9 probe differentiated the cms line 81 A4, derived from P. glaucum subsp. monodii, while the coxII gene probe did not detect any polymorphism among the cms lines studied. MtDNA digested with BamHI, PstI or XhoI and hybridized to the atp6 probe revealed distinct differences among the cms lines. The maize atp6 gene clone identified four distinct cytoplasmic groups and four subgroups within a main group. The mtDNA fragments hybridized to the atp6 gene probe with differing intensities, suggesting the presence of more than one copy of the gene in different stoichiometries. Rearrangements involving the coxI and/or rrn18-rrn5 genes (mapped within the pearl millet clones) probably resulted in the S-A1 type of sterility. Rearrangements involving the atp6 gene (probably resulting in chimeric form) may be responsible for male sterility in other cms lines of pearl millet.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Mitochondrial DNA . Cytoplasmic male sterility Pearl millct
Subjects:	Mandate crops > Millets
Depositing User:	Mr Charan Sai Ch
Date Deposited:	23 Oct 2011 09:44
Last Modified:	23 Oct 2011 09:44
URI:	http://oar.icrisat.org/id/eprint/3135
Official URL:	http://dx.doi.org/10.1007/BF00223658
Projects:	UNSPECIFIED
Funders:	UNSPECIFIED
Acknowledgement:	We sincerely thank Drs. S. Appa Rao and K. N. Rai. ICRISAT, for providing us with the necessary seed malerial. We thank Mr. D. Midya. ICRISAT for help in cluster analyses. We acknowledge the helpof Mr. R. Luke in the labexperiments. International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) Journal Article No. 1416.
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