Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India

Reddy, D V R and Ratna, A S and Sudarshana, M R and Poul, F and Kumar, I K (1992) Serological relationships and purification of bud necrosis virus, a tospovirus occurring in peanut (Arachis hypogaea L.) in India. Annals of Applied Biology, 120 (2). pp. 279-286. ISSN 1744-7348

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Abstract

A procedure for the purification of a tospovirus which causes bud necrosis disease (BND) of peanut in India is described. The virus contained three polypeptides of 78 kDa, 54 kDa and 31 kDa. In two ELISA procedures the virus failed to react with antisera to tomato spotted wilt virus (TSWV) obtained from different sources and with an antiserum to impatiens necrotic spot virus (INSV). Additionally, in reciprocal tests TSWV and INSV antigens failed to react with antiserum to the virus infecting peanut in India. In electro-blot immunoassay 54 kDa and 31 kDa polypeptides of the virus reacted with the homologous antiserum. None of the heterologous antisera reacted with any of the three viral polypeptides. On the basis of serological differences the virus that causes BND in India is distinct and therefore has been named bud necrosis virus (BNV). This serotype appears to be restricted to Asia.

Item Type: Article
Divisions: UNSPECIFIED
CRPS: UNSPECIFIED
Uncontrolled Keywords: Bud necrosis virus, tomato spotted wilt virus, impatiens nccrotic spot virus, ELISA, electro-blot immunoassay, serotypes, tospoviruscs
Subjects: Mandate crops > Groundnut
Depositing User: Mr Charan Sai Ch
Date Deposited: 22 Oct 2011 09:06
Last Modified: 22 Oct 2011 09:06
URI: http://oar.icrisat.org/id/eprint/3101
Official URL: http://dx.doi.org/10.1111/j.1744-7348.1992.tb03425...
Projects: UNSPECIFIED
Funders: UNSPECIFIED
Acknowledgement: We are grateful to Dr D. McDonald for his many valuable comments on the manuscript and to Dr G. Adam for the supply of antiserum to the tospovirus isolated from watermclon.
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