Genetic variability characterisation of Tanzania sorghum landraces based on simple sequence repeats (SSRS) molecular and morphological markers

    Bucheyeki, T L and Gwanama, C and Mgonja, M and Chisi, M and Folkertsma, R and Mutegi, R (2009) Genetic variability characterisation of Tanzania sorghum landraces based on simple sequence repeats (SSRS) molecular and morphological markers. African Crop Science Journal, 17 (2). pp. 71-86.

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    Abstract

    Morphological and molecular markers were employed to study the genetic variability among 40 sorghum landraces collected from Tanzania and two from Zambia. A total of 14 morphological markers and seven simple sequence repeats (SSRs) primers were used to (i) determine the genetic relationships among landraces, and (ii) assess important agronomic traits. Five principal components accounted for 73.60% of the total variability. There were positive significant correlation between yield and inflorescence width (r = 0.343*), five panicles averages and plot yields (r = 0. 531*), leaf senescence and inflorescence length (r = 0.355*). Negative significant correlation between tiller numbers and inflorescence length (r = -0.343*) was recorded. Cluster analysis based on morphological traits revealed three major distinct groups with one landrace forming independent cluster. Based on molecular markers, eleven clusters were observed. Sorghum controls, N13, Ochuti and Adiwo each formed independent clusters. Markers Xgap84 and Xtxp320 had high alleles than other markers. These had seven and eight alleles respectively. Molecular markers clearly separated landraces within and between groups than morphological markers. There were no genotype specific makers with the exceptions of Ochuti and N13 controls.

    Item Type: Article
    Divisions: UNSPECIFIED
    CRP: UNSPECIFIED
    Subjects: Mandate crops > Sorghum
    Depositing User: Library ICRISAT
    Date Deposited: 29 Aug 2011 04:32
    Last Modified: 29 Aug 2011 04:32
    URI: http://oar.icrisat.org/id/eprint/302
    Official URL:
    Projects: UNSPECIFIED
    Funders: UNSPECIFIED
    Acknowledgement: UNSPECIFIED
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