Generation and exploitation of EST-derived SSR markers for assaying molecular diversity in durum wheat populations

Chabane, K and Varshney, R K and Graner, A and Valkoun, J (2008) Generation and exploitation of EST-derived SSR markers for assaying molecular diversity in durum wheat populations. Genetic Resources and Crop Evolution, 55 (6). pp. 869-881. ISSN 1573-5109

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Abstract

Durum wheat [Triticum turgidum L. subsp. turgidum convar. durum (Desf.) MK] is an important cereal crop economically and nutritionally in the Central Asia and Caucasian, West Asia, and North Africa (CWANA) regions. Durum landraces and improved lines are largely grown in this region. Its genetic diversity has been studied using different molecular markers. The increasing availability of expressed sequence tags (ESTs) in wheat (Triticum aestivum) and related cereals provides a valuable resource of non-anonymous DNA markers to study durum diversity. In this study, a set of 517,319 Triticum aestivum EST sequences was employed for the identification of wheat simple sequence repeats called microsatellites (W-eSSRs) with the help of a PERL5 script called MISA. In comparison, barley microsatellites (B-eSSRs) have been used to exploit their transferability to durum wheat. Newly developed W-eSSR markers were probed on the 115 recombinant inbred lines (RIL) of the International Triticeae Mapping Initiative (ITMI) population (Opata 85 × Synthetic 7984). The polymorphic eSSRs were mapped. To examine the potential of the two types of eSSRs markers, 12 W-eSSR markers and 13 B-eSSR markers were used to fingerprint 153 wheat genotypes. Our results indicate that: (1) B-eSSRs show a high level of transferability to wheat, (2) the developed W-eSSRs are significantly polymorphic than those derived from genomic regions, (3) new W-eSSRs were identified and integrated in the ITMI genetic linkage map and, (4) B-eSSR and W-eSSRs are providing additional markers for comparative mapping following gene introgressions from wild species and carrying out evolutionary studies.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	B-eSSRs. ESTs, Genetic diversity, Genetic linkage mapping, Polymorphism Information Content (PIC), W-eSSRs
Subjects:	Others > Wheat
Depositing User:	Library ICRISAT
Date Deposited:	21 Oct 2011 11:12
Last Modified:	21 Oct 2011 11:12
URI:	http://oar.icrisat.org/id/eprint/3008
Official URL:	http://dx.doi.org/10.1007/s10722-007-9292-8
Projects:	Exploration of Genetic Resources Collections at ICARDA for Adaptation to Climate Change: Identification and Utilization of Sources of Stress Tolerance
Funders:	German Federal Ministry of Economic Cooperation and Development-ICARDA
Acknowledgement:	The authors’ research was supported by grants to ICARDA from the German Federal Ministry of Economic Cooperation and Development (BMZ, Bonn, Germany) under the project “Exploration of Genetic Resources Collections at ICARDA for Adaptation to Climate Change: Identification and Utilization of Sources of Stress Tolerance”. We thank Dr. W. Choumane (Teschrine University, Lattakia, Syria), Dr. M. Nachit (durum wheat breeder, ICARDA) and Dr. K. Amar (durum wheat breeder, CIMMYT) for their comments and reviewing the manuscript; and Dr. J. Rayan and Mr. A. Varadachary for editing the paper.
Links:	Author

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