Sreenivasulu, P and Demski, J W and Reddy, D V R and Naidu, R A and Ratna, A S (1991) Purification and some serological relationships of tomato spotted wilt virus isolates occurring on peanut (Arachis hypogaea) in the USA. Plant Pathology, 40 (4). pp. 503-507.
![[img]](http://oar.icrisat.org/style/images/fileicons/application_pdf.png)
|
PDF
Download (329kB) | Preview |
Abstract
A procedure for the purification of TSWV-Tx, a tomato spotted wilt virus isolate infecting groundnuts in Texas, is described. A rabbit antiserum was produced. Several TSWV isolates occurring on groundnuts in the USA reacted to varying extents in ELISA with antisera to TSWV-Tx and to Greek TSWV isolates, but failed to react with antiserum to an isolate of TSWV from India. In reciprocal tests, antigens of the Indian TSWV failed to react with antisera to Tx and to the Greek isolates. Purified TSWV-Tx contained 4 polypeptide species of 78 000, 54 000 and 27 000 Da. In electro-blot immunoassays, all 4 polypeptides reacted with the homologous antiserum and with antisera prepared against a Greek, a Dutch and an Australian isolate. None of the polypeptides reacted with the antiserum to the Indian isolate.
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Others > Plant Pathology |
| Depositing User: | Library ICRISAT |
| Date Deposited: | 28 Oct 2011 04:08 |
| Last Modified: | 28 Oct 2011 04:08 |
| URI: | http://oar.icrisat.org/id/eprint/2764 |
| Official URL: | |
| Projects: | UNSPECIFIED |
| Funders: | UNSPECIFIED |
| Acknowledgement: | UNSPECIFIED |
| Links: |
Actions (login required)
 |
View Item |
Altmetric
Altmetric