Complementary DNA probe for the detection of isolates of Indian peanut clump virus

Balan, K V P (1989) Complementary DNA probe for the detection of isolates of Indian peanut clump virus. Masters thesis, Indian Institute of Technology Kharagpur, West Bengal.

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Supervisors

Supervisors Name	Supervisors ID
Jana, M K	IIT Kharagpur
Reddy, D V R	ICRISAT

Abstract

The main aim of the present study was to use the clones of PCV genome and select a clone which would hybridize with RNA of several PCV isolates which could be used in the preparation of a C-DNA probe. For this purpose Northern Dot Blot hybridization technique was used which was probed with nick translated 32 p. labeled C-DNA clones of H-IPCV. Among the H-IPCV clines analyzed the 2 kbp and 3.5-4 kbp clines were employed in this study to probe the IPCV isolates. Autoradiographic signals showed greater hybridization could be achieved with 3.5-4 kbp clone than 2 kbp clone using the pure virus and nucleic acid of H-IPCV and with RNA of partially purified preparations of L-IPCV and total nucleic acids of B-IPCV isolates

Item Type:	Thesis (Masters)
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Subjects:	Mandate crops > Groundnut
Depositing User:	Mr T L Gautham
Date Deposited:	17 Oct 2011 06:37
Last Modified:	20 Oct 2011 05:11
URI:	http://oar.icrisat.org/id/eprint/2666
Acknowledgement:	UNSPECIFIED
Links:	Author

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