Genetic diversity among pearl millet maintainers using microsatellite markers

Kapila, R K and Yadav, R S and Plaha, P and Rai, K N and Yadav, O P and Hash, C T and Howarth, C J (2008) Genetic diversity among pearl millet maintainers using microsatellite markers. Plant Breeding, 127 (1). pp. 33-37.

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Abstract

Genetic diversity among 70 maintainers and two pollinators of sub-Saharan and Indian origin was studied for simple sequence repeat (SSR) loci using 34 primer pairs. A total of 213 alleles were detected with an average of 6.26 alleles per locus. Polymorphic information content (PIC) ranged from 0.05 to 0.96 with a mean of 0.58 for the SSR loci. Mean PIC across the linkage groups and number of alleles in dinucleotide motifs varied significantly. The lowest PIC (0.239) for linkage group 6 indicated comparatively conserved nature of this linkage group. Genetic similarity estimates ranged from 0.05 to 0.73 with an average value of 0.29. This indicated sufficient diversity among the maintainer and pollinator lines. The 72 lines fell in five clusters, and the clustering pattern corroborated with their pedigree and characteristic traits. Pollinator ICMR 356 was more diverse from the maintainer lines analysed, and can be a potential parent for pearl millet hybrid development.

Item Type:	Article
Divisions:	UNSPECIFIED
CRP:	UNSPECIFIED
Uncontrolled Keywords:	Pennisetum glaucum, genetic diversity, SSR, ICRISAT
Subjects:	Mandate crops > Millets
Depositing User:	Mr Sanat Kumar Behera
Date Deposited:	15 Oct 2011 10:54
Last Modified:	15 Oct 2011 10:54
URI:	http://oar.icrisat.org/id/eprint/2636
Official URL:	http://dx.doi.org/10.1111/j.1439-0523.2007.01433.x
Projects:	UNSPECIFIED
Funders:	Government of India - BOYSCAST felloship, Royal Society , Indian Social Science Academy, Department for International Development
Acknowledgement:	We thank Dr MS Dhanoa and Dr Ruth Sanderson for providing help in statistical analysis of data. RK Kapila acknowledges the HP Agricultural University, Palampur, India for granting study leave and the Department of Science & Technology, Government of India for providing BOYSCAST Fellowship. OP Yadav acknowledges funding from the Royal Society and Indian National Science Academy. This document is an output from a project (Plant Sciences research Programme R6451) funded by the UK Department for International Development (DFID) and administered by the Centre for Arid Zone Studies (CAZS) for the benefit of developing countries. The views expressed are not necessarily those of the DFID.
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