Delorme, V and Keen, C L and Rai, K N and Leaver, C J (1997) Cytoplasmic-nuclear male sterility in pearl millet: comparative RFLP and transcript analyses of isonuclear male-sterile lines. TAG Theoretical and Applied Genetics, 95 (5-6). pp. 961-968. ISSN 1432-2242
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Abstract
The identification of diagnostic cytoplasmic molecular markers is of prime interest to pearl millet breeders wishing to identify sources of cytoplasmic-nuclear male sterility (CMS) which can be used as an alternative to the single source currently used in the production of F1 hybrid seed. Here, we report the classification of five pearl millet CMS sources based on RFLP analysis of isonuclear lines carried out using mitochondrial gene-specific DNA probes in combination with eight restriction endonucleases. On the basis of RFLP data, the five CMS cytoplasms can be distinguished from each other and from the isonuclear fertile cytoplasm. In addition, based on cox1, cox3, atp6 and atp9 polymorphisms, these lines can be classified into two major groups: one corresponds to A5, Aegp, Av and A1 cytoplasms, and the other consists of the A4 cytoplasm. Our results suggest that a rearrangement involving the cox1 gene might be related to CMS in the first group (A5, Aegp, Av and A1), whereas a rearrangement within the atp6/cox3 cluster region might be related to CMS in the second group (A4).
| Item Type: | Article |
|---|---|
| Divisions: | UNSPECIFIED |
| CRP: | UNSPECIFIED |
| Subjects: | Mandate crops > Millets |
| Depositing User: | Mr Sanat Kumar Behera |
| Date Deposited: | 15 Oct 2011 08:37 |
| Last Modified: | 15 Oct 2011 08:37 |
| URI: | http://oar.icrisat.org/id/eprint/2609 |
| Official URL: | http://dx.doi.org/10.1007/s001220050648 |
| Projects: | Centre for Arid Zone Studies-Plant Sciences Research Programme |
| Funders: | Overseas Development Administration |
| Acknowledgement: | We thank Drs D. R. Pring and W. W. Hauswirth (University of Florida, Gainesville, USA) and A. Brennicke (Institut fur Genbiologische Forschung, Berlin, Germany) for the gift of mtDNA probes and John Baker for photographic work. This document is an output from a project funded by the Overseas Development Administration (ODA) Plant Sciences Research Pro gramme, managed by the Centre for Arid Zone Studies, University of Wales, Bangor, for the benefit of developing countries. The views expressed are not necessarily those of the ODA. |
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